[1] Shen Jiayao, Xiao Pengfeng, Hou Peng, Ji Meiju, et al. Assembly fabrication of linkers on glass surface andtheir effect on DNA synthesis and hybridization [J]. Journal of Southeast University (English Edition), 2003, 19 (2): 149-154. [doi:10.3969/j.issn.1003-7985.2003.02.011]
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Assembly fabrication of linkers on glass surface andtheir effect on DNA synthesis and hybridization(
)
玻片表面手臂分子的组装及 对寡核苷酸原位合成与杂交的影响
)Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]
- Volumn:
- 19
- Issue:
- 2003 2
- Page:
- 149-154
- Research Field:
- Chemistry and Chemical Engineering
- Publishing date:
- 2003-06-30
Info
- Title:
- Assembly fabrication of linkers on glass surface andtheir effect on DNA synthesis and hybridization
- 玻片表面手臂分子的组装及 对寡核苷酸原位合成与杂交的影响
- Author(s):
- Shen Jiayao; Xiao Pengfeng; Hou Peng; Ji Meiju; Sun Xiao; He Nongyue
- Department of Biological Science and Medical Engineering, Chien-shiung Wu Laboratory, Southeast University, Nanjing 210096, China
- Keywords:
- linker; assembly fabrication; in situ oligonucleotide; synthesis; hybridization
- PACS:
- O633
- DOI:
- 10.3969/j.issn.1003-7985.2003.02.011
- Abstract:
- Linkers were assembled on a glass surface based on the hydrolysis and condensation of 3-glycidoxypropyltrimethoxysilane(GPS). After the assembly of GPS, four approaches were tried to open the ending epoxide group of GPS or to further elongate the linkers. The effect of these approaches on DNA in situ synthesis and hybridization was investigated. For the spacing of the synthesis initiation sites, the wettability of the support and the length of the linking group that attaches the initiation site to the surface have direct influences on the yield of coupling reactions and the subsequent hybridization events. X-ray photoelectron spectroscopy(XPS)and mean contact angles of deionized water of the above slides were measured to assess the linker’s characteristics in each procedure. It was proved that the glass slides were successfully modified and became excellent supports for the oligonucleotides synthesis. In addition, it proved best for the in situ oligonucleotides synthesis that a glass slide was in turn treated with ethylenediamine, glutaradehyde, ethanolamine and sodium borohydride solution at ambient temperature after silanized with GPS.
- 以3-缩水甘油-环氧丙基-三甲氧基硅烷对玻璃基片表面进行硅烷化后, 用下列4种方法组装手臂分子:(1) 盐酸直接处理;(2) 先用聚六乙二醇, 然后用盐酸处理;(3) 用乙二胺、戊二醛、乙醇胺和硼氢化钠分别处理;(4) 用聚六乙二醇、乙二胺、戊二醛、乙醇胺和硼氢化钠分别处理.用XPS(X-ray photoelectron spectroscopy)和测定接触角的方法对上述组装进行了表征, 并用直接偶联荧光单体及合成20 mer寡核苷酸与带荧光的互补探针杂交的方法对上述手臂分子的合成效率及杂交效率进行了考
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Memo
- Memo:
- Biographies: Shen Jiayao(1979—), female, graduate; He Nongyue(corresponding author), male, professor, nyhe@seu.edu.cn.