[1] Shen Jiayao, Xiao Pengfeng, Hou Peng, Ji Meiju, et al. Assembly fabrication of linkers on glass surface andtheir effect on DNA synthesis and hybridization [J]. Journal of Southeast University (English Edition), 2003, 19 (2): 149-154. [doi:10.3969/j.issn.1003-7985.2003.02.011]

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Assembly fabrication of linkers on glass surface andtheir effect on DNA synthesis and hybridization()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

19

Issue:

2003 2

Page:

149-154

Research Field:

Chemistry and Chemical Engineering

Publishing date:

2003-06-30

Info

Title:

Assembly fabrication of linkers on glass surface andtheir effect on DNA synthesis and hybridization

Author(s):

Shen Jiayao;  Xiao Pengfeng;  Hou Peng;  Ji Meiju;  Sun Xiao;  He Nongyue

Department of Biological Science and Medical Engineering, Chien-shiung Wu Laboratory, Southeast University, Nanjing 210096, China

Keywords:

linker;  assembly fabrication;  in situ oligonucleotide;  synthesis;  hybridization

PACS:

O633

DOI:

10.3969/j.issn.1003-7985.2003.02.011

Abstract:

Linkers were assembled on a glass surface based on the hydrolysis and condensation of 3-glycidoxypropyltrimethoxysilane(GPS). After the assembly of GPS, four approaches were tried to open the ending epoxide group of GPS or to further elongate the linkers. The effect of these approaches on DNA in situ synthesis and hybridization was investigated. For the spacing of the synthesis initiation sites, the wettability of the support and the length of the linking group that attaches the initiation site to the surface have direct influences on the yield of coupling reactions and the subsequent hybridization events. X-ray photoelectron spectroscopy(XPS)and mean contact angles of deionized water of the above slides were measured to assess the linker’s characteristics in each procedure. It was proved that the glass slides were successfully modified and became excellent supports for the oligonucleotides synthesis. In addition, it proved best for the in situ oligonucleotides synthesis that a glass slide was in turn treated with ethylenediamine, glutaradehyde, ethanolamine and sodium borohydride solution at ambient temperature after silanized with GPS.

References:

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Memo

Memo:

Biographies: Shen Jiayao(1979—), female, graduate; He Nongyue(corresponding author), male, professor, nyhe@seu.edu.cn.

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