[1] Pan Haixian, Lü Xiaoying, Wang Zhigong, Fang Tao, et al. Microelectrode array for bioelectrical signal stimulation and recording [J]. Journal of Southeast University (English Edition), 2011, 27 (4): 361-366. [doi:10.3969/j.issn.1003-7985.2011.04.003]

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Microelectrode array for bioelectrical signal stimulation and recording()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

27

Issue:

2011 4

Page:

361-366

Research Field:

Circuit and System

Publishing date:

2011-12-31

Info

Title:

Microelectrode array for bioelectrical signal stimulation and recording

Author(s):

Pan Haixian¹;  Lü Xiaoying¹;  Wang Zhigong²;  Fang Tao¹;  Qiu Lei²;  Huang Zonghao²

¹ State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
² Institute of RF- & OE-ICs, Southeast University, Nanjing 210096, China

Keywords:

microelectrode array(MEA);  stimulation and recording;  extracellular recording;  toad sciatic nerve

PACS:

TN43

DOI:

10.3969/j.issn.1003-7985.2011.04.003

Abstract:

A microelectrode array(MEA)is presented, which is composed of 60 independent electrodes with 59 working ones and one reference one, and they are divided into 30 pairs. Except for the reference electrode, each pair consists of one stimulating electrode and one recording electrode. Supported by the peripheral circuits, four electrode states to study the bioelectrical signal of biological tissue or slice cultured in-vitro on the surface of the electrodes can be realized through each pair of electrodes. The four electrode states are stimulation, recording, stimulation and recording simultaneously, and isolation. The state of each pair of working electrodes can be arbitrarily controlled according to actual needs. The MEAs are fabricated in printed circuit board(PCB)technology. The total area of the PCB-based MEA is 49 mm×49 mm. The impedance measurement of MEA is carried out in 0.9% sodium chloride solution at room temperature by means of 2-point measurements with an Agilent LCR meter, and the test signal for the impedance measurement is sinusoidal(AC voltage 50 mV, sweeping frequency 20 Hz to 10 kHz). The electrode impedance is between 200 and 3 kΩ while the frequency is between 500 and 1 000 Hz. The electrode impedance magnitude is inversely proportional to the frequency. Experiments of toad sciatic nerve in-vitro stimulation and recording and signal regeneration between isolated toad sciatic nerves are carried out on the PCB-based MEA. The results show that the MEA can be used for bioelectrical signal stimulation, recording, stimulation and recording simultaneously, and isolation of biological tissues or slices in-vitro.

References:

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Memo

Memo:

Biographies: Pan Haixian(1983—), female, graduate; Lü Xiaoying(corresponding author), female, doctor, professor, luxy@seu.edu.cn.
Foundation items: The National Natural Science Foundation of China(No.61076118, 90307013, 90707005), the Natural Science Foundation of Jiangsu Province(No.BK2008032), Special Foundation and Open Foundation of the State Key Laboratory of Bioelectronics of Southeast University.
Citation: Pan Haixian, Lü Xiaoying, Wang Zhigong, et al.Microelectrode array for bioelectrical signal stimulation and recording[J].Journal of Southeast University(English Edition), 2011, 27(4):361-366.[doi:10.3969/j.issn.1003-7985.2011.04.003]

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