[1] Wei HaiyanYang Fei, Yin LihongPu Yuepu,. Effects and mechanisms of L-phenylalanineon growth of Microcystis aeruginosa [J]. Journal of Southeast University (English Edition), 2011, 27 (4): 445-448. [doi:10.3969/j.issn.1003-7985.2011.04.019]

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Effects and mechanisms of L-phenylalanineon growth of Microcystis aeruginosa()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

27

Issue:

2011 4

Page:

445-448

Research Field:

Environmental Science and Engineering

Publishing date:

2011-12-31

Info

Title:

Effects and mechanisms of L-phenylalanineon growth of Microcystis aeruginosa

Author(s):

Wei HaiyanYang Fei;  Yin LihongPu Yuepu

Key Laboratory of Environmental Medicine Engineering of Ministry of Education, Southeast University, Nanjing 210009, China

Keywords:

algae control;  L-phenylalanine;  mechanisms;  flow cytometry;  membranes;  growth

PACS:

X506

DOI:

10.3969/j.issn.1003-7985.2011.04.019

Abstract:

The effects and possible mechanisms of action of L-phenylalanine on the growth of Microcystis aeruginosa cells were explored by cell counting and flow cytometry assays. L-phenylalanine promoted the growth of Microcystis aeruginosa at concentrations between 0.078 and 0.312 μg/mL, but inhibited growth at concentrations between 0.625 and 20 μg/mL in 24 h exposure. The dose-effect and time-course relationships between exposure to L-phenylalanine and growth inhibition of Microcystis aeruginosa were observed. The IC₅₀ value of L-phenylalanine for growth inhibition of Microcystis aeruginosa was 6.2 μg/mL(95% confidence interval was 0.005 to 16.76 μg/mL). The membrane integrity of the cells showed significant variations after 24 h exposure to L-phenylalanine. Meanwhile, no effects on esterase activity of the cells were observed until after 48 h exposure to L-phenylalanine. In conclusion, L-phenylalanine has hormesis effects and algae control effects on Microcystis aeruginosa. The latter is closely related to alterations or disorders in the cell membrane and with variation of esterase activity in the cells.

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Memo

Memo:

Biographies: Wei Haiyan(1987—), female, graduate; Pu Yuepu(corresponding author), male, doctor, professor, yppu@seu.edu.cn.
Foundation items: The National Science and Technology Major Project(No.2009ZX07101-011), the National Natural Science Foundation of China(No.30972440).
Citation: Wei Haiyan, Yang Fei, Yin Lihong, et al.Effects and mechanisms of L-phenylalanine on growth of Microcystis aeruginosa[J].Journal of Southeast University(English Edition), 2011, 27(4):445-448.[doi:10.3969/j.issn.1003-7985.2011.04.019]

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