[1] Xu Yan, Lam Ka-Ho, Lam Michael Hon-Wah, et al. Gene expression profile in H4IIE rat hepatoma cells exposed toan antifouling booster biocide Irgarol-1051 degradation product [J]. Journal of Southeast University (English Edition), 2014, 30 (4): 506-513. [doi:10.3969/j.issn.1003-7985.2014.04.018]

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Gene expression profile in H4IIE rat hepatoma cells exposed toan antifouling booster biocide Irgarol-1051 degradation product()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

30

Issue:

2014 4

Page:

506-513

Research Field:

Environmental Science and Engineering

Publishing date:

2014-12-31

Info

Title:

Gene expression profile in H4IIE rat hepatoma cells exposed toan antifouling booster biocide Irgarol-1051 degradation product

Author(s):

Xu Yan¹;  2;  Lam Ka-Ho²;  Lam Michael Hon-Wah²

¹School of Civil Engineering, Southeast University, Nanjing 210096, China
²Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China

Keywords:

Irgarol-1051;  degradation product M2;  microarray;  gene expression;  genotoxicity

PACS:

X55

DOI:

10.3969/j.issn.1003-7985.2014.04.018

Abstract:

To better understand the toxicity of an antifouling booster biocide Irgarol-1051 degradation product M2(3-［4-tert-butylamino-6-methylthiol-s-triazin-2-ylamino］pro-pionaldehyde), this study utilized a DNA microarray technique to explore the genotoxicity of M2. The Affymetrix, Inc. rat genome 230 2.0 GeneChip was employed to examine alterations in gene regulation in rat hepatoma cells exposed to 30 μmol/L of M2 for 96 h. The results showed that 38 genes were significantly(p<0.002 5)altered by M2 at two-fold changes in all the four possible control/exposure comparisons. Accn5 was the only well described gene consistently being suppressed, which likely altered the epithelial sodium channel(ENaC). 10 and 82 annotated genes were up- and down-regulated in at least one of the control/exposure comparisons, respectively. The induced genes were mainly involved in the nucleus belonging to the cellular component. The largest categories of suppression concerned G-protein coupled receptor protein signaling pathways belonging to the biological process and integral to membranes belonging to the cellular component.

References:

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Memo

Memo:

Biography: Xu Yan(1980—), female, doctor, lecturer, xuxucalmm@seu.edu.cn.
Foundation items: The Research Grants Council of Hong Kong SAR, China(No.CityU, 1445/05M), the National Natural Science Foundation of China(No.41301546).
Citation: Xu Yan, Lam Ka-Ho, Lam Michael Hon-Wah.Gene expression profile in H4IIE rat hepatoma cells exposed to an antifouling booster biocide Irgarol-1051 degradation product[J].Journal of Southeast University(English Edition), 2014, 30(4):506-513.[doi:10.3969/j.issn.1003-7985.2014.04.018]

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