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[1] Xu Yan, Lam Ka-Ho, Lam Michael Hon-Wah, et al. Gene expression profile in H4IIE rat hepatoma cells exposed toan antifouling booster biocide Irgarol-1051 degradation product [J]. Journal of Southeast University (English Edition), 2014, 30 (4): 506-513. [doi:10.3969/j.issn.1003-7985.2014.04.018]
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Gene expression profile in H4IIE rat hepatoma cells exposed toan antifouling booster biocide Irgarol-1051 degradation product()
防污损涂料添加剂Irgarol-1051降解产物对H4IIE 鼠肝癌细胞的基因影响
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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:
30
Issue:
2014 4
Page:
506-513
Research Field:
Environmental Science and Engineering
Publishing date:
2014-12-31

Info

Title:
Gene expression profile in H4IIE rat hepatoma cells exposed toan antifouling booster biocide Irgarol-1051 degradation product
防污损涂料添加剂Irgarol-1051降解产物对H4IIE 鼠肝癌细胞的基因影响
Author(s):
Xu Yan1;  2;  Lam Ka-Ho2;  Lam Michael Hon-Wah2
1School of Civil Engineering, Southeast University, Nanjing 210096, China
2Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China
许妍1;  2;  林家豪2;  林汉华2
1东南大学土木工程学院, 南京 210096; 2香港城市大学生物与化学系, 中国香港
Keywords:
Irgarol-1051;  degradation product M2;  microarray;  gene expression;  genotoxicity
Irgarol-1051;  降解产物M2;  微阵列;  基因表达;  基因毒性
PACS:
X55
DOI:
10.3969/j.issn.1003-7985.2014.04.018
Abstract:
To better understand the toxicity of an antifouling booster biocide Irgarol-1051 degradation product M2(3-［4-tert-butylamino-6-methylthiol-s-triazin-2-ylamino］pro-pionaldehyde), this study utilized a DNA microarray technique to explore the genotoxicity of M2. The Affymetrix, Inc. rat genome 230 2.0 GeneChip was employed to examine alterations in gene regulation in rat hepatoma cells exposed to 30 μmol/L of M2 for 96 h. The results showed that 38 genes were significantly(p<0.002 5)altered by M2 at two-fold changes in all the four possible control/exposure comparisons. Accn5 was the only well described gene consistently being suppressed, which likely altered the epithelial sodium channel(ENaC). 10 and 82 annotated genes were up- and down-regulated in at least one of the control/exposure comparisons, respectively. The induced genes were mainly involved in the nucleus belonging to the cellular component. The largest categories of suppression concerned G-protein coupled receptor protein signaling pathways belonging to the biological process and integral to membranes belonging to the cellular component.
为研究海洋防污损涂料添加剂Irogarol-1051的降解产物M2的基因毒性, 应用微阵列技术, 选取Affymetrix公司鼠基因组230 2.0基因芯片检测30 μmol/L M2暴露下的鼠肝癌细胞基因表达变化.实验结果显示, 96 h的M2暴露导致了38个基因在全部4组可能的对照/暴露中均发生显著变化(p<0.002 5), 其中只有Accn5基因研究较为透彻, 该基因表达的抑制可能影响上皮钠通道的功能.此外, 分别有10和82个功能注释基因在至少一组对照/暴露组中上调和下调.M2诱导的基因主要和细胞核(细胞成分)相关.M2抑制的基因则主要影响生物过程中的G蛋白偶联信号通路功能和细胞成分中的细胞膜内整合功能.

References:

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Memo

Memo:
Biography: Xu Yan(1980—), female, doctor, lecturer, xuxucalmm@seu.edu.cn.
Foundation items: The Research Grants Council of Hong Kong SAR, China(No.CityU, 1445/05M), the National Natural Science Foundation of China(No.41301546).
Citation: Xu Yan, Lam Ka-Ho, Lam Michael Hon-Wah.Gene expression profile in H4IIE rat hepatoma cells exposed to an antifouling booster biocide Irgarol-1051 degradation product[J].Journal of Southeast University(English Edition), 2014, 30(4):506-513.[doi:10.3969/j.issn.1003-7985.2014.04.018]
Last Update: 2014-12-20