[1] Cai Di, Kan Yajing, Zhao Gutian, Wu Gensheng, et al. Adsorption and desorption behaviors of ssDNA moleculeson mica surface by surface forces apparatus [J]. Journal of Southeast University (English Edition), 2016, 32 (1): 45-50. [doi:10.3969/j.issn.1003-7985.2016.01.009]

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Adsorption and desorption behaviors of ssDNA moleculeson mica surface by surface forces apparatus()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

32

Issue:

2016 1

Page:

45-50

Research Field:

Mathematics, Physics, Mechanics

Publishing date:

2016-03-20

Info

Title:

Adsorption and desorption behaviors of ssDNA moleculeson mica surface by surface forces apparatus

Author(s):

Cai Di;  Kan Yajing;  Zhao Gutian;  Wu Gensheng;  Si Wei;  Tan Qiyan;  Chen Yunfei

School of Mechanical Engineering, Southeast University, Nanjing 211189, China

Keywords:

single-stranded DNA;  mica;  adsorption;  desorption;  competition effect;  surface forces apparatus

PACS:

O561.4

DOI:

10.3969/j.issn.1003-7985.2016.01.009

Abstract:

An approach for studying the adsorption and desorption behaviors of single-stranded DNA(ssDNA)molecules on the mica surface by the surface forces apparatus(SFA)is reported, which can be used to characterize the precise thickness, configuration and mechanical properties of ssDNA layers on the mica surface at a certain buffer solution. The formation of ssDNA layers is first studied by tuning the ssDNA concentrations, and the experimental results indicate that the ssDNA concentration of 100 ng/μL is ideal for forming a ssDNA monolayer structure on the mica surface, and the hardwall value measured to be 1.04 nm under this circumstance is regarded as the thickness of the ssDNA monolayer confined on mica. The desorption behavior of ssDNA molecules from the mica surface is further studied by observing and comparing different shapes of the force-distance curves under certain conditions. It is found that the desorption of ssDNA molecules from the mica surface occurs as the monovalent salts are added into the gap buffer. It is inferred that the competition effect between monovalent and divalent salts can induce the release of ssDNA from substrate. The results also reveal that 10 mmol/L monovalent salts(Na⁺)is sufficient for the desorption of ssDNA from mica. This work provides an applicable method to study the binding mechanism of ssDNA molecules on inorganic substrates.

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Memo

Memo:

Biographies: Cai Di(1990—), male, graduate; Chen Yunfei(corresponding author), male, doctor, professor, yunfeichen@seu.edu.cn.
Foundation items: The National Basic Research Program of China(973 Program)(No.2011CB707605), the Fundamental Research Funds for the Central Universities(No.2242015K42085), the Scientific Innovation Research of College Graduates in Jiangsu Province(No.KYLX_-0100), the Research Start-Up Fund of Southeast University.
Citation: Cai Di, Kan Yajing, Zhao Gutian, et al. Adsorption and desorption behaviors of ssDNA molecules on mica surface by surface forces apparatus[J].Journal of Southeast University(English Edition), 2016, 32(1):45-50.DOI:10.3969/j.issn.1003-7985.2016.01.009.

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