[1] Yu Jingwen, Si Wei, Sha Jingjie, Chen Yunfei, et al. Effect of salt gradients on DNA translocationthrough solid-state nanopores [J]. Journal of Southeast University (English Edition), 2016, 32 (3): 307-311. [doi:10.3969/j.issn.1003-7985.2016.03.008]

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Effect of salt gradients on DNA translocationthrough solid-state nanopores()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

32

Issue:

2016 3

Page:

307-311

Research Field:

Mechanical Engineering

Publishing date:

2016-09-20

Info

Title:

Effect of salt gradients on DNA translocationthrough solid-state nanopores

Author(s):

Yu Jingwen;  Si Wei;  Sha Jingjie;  Chen Yunfei

School of Mechanical Engineering, Southeast University, Nanjing 211189, China
Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing 211189, China

Keywords:

solid-state nanopore;  salt gradients;  λ-DNA;  signal-to-noise ratio;  current blockade;  dwell time

PACS:

TH789;Q786

DOI:

10.3969/j.issn.1003-7985.2016.03.008

Abstract:

Aiming at the issues of controlling the translocation speed of DNA through a solid-state nanopore and enlarging the signal-to-noise ratio of ionic current modulation, which are challenges for the application of nanopore technology in DNA detection, salt concentration gradients are applied across the nanopore to investigate their influence on the DNA translocation time and signal-to-noise ratio. Experimental data demonstrates that, in symmetric concentration conditions, both the current blockade and dwell time for λ-DNA translocation through a solid-state nanopore increase along with potassium chloride concentration. When the concentration in the trans chamber is decreased from 1 to 0.1 mol/L, keeping the concentration of the cis chamber at 1 mol/L, the normalized current blockade is found to be increased by one order. The increased dwell time and enhanced signal-to-noise ratio are achieved with salt gradients across the nanopore, which can improve the sensitivity when detecting DNA samples.

References:

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Memo

Memo:

Biographies: Yu Jingwen(1992—), female, graduate; Chen Yunfei(corresponding author), male, doctor, professor, yunfeichen@seu.edu.cn.
Foundation items: The National Natural Science Foundation of China(No. 51435003, 51375092), Fundamental Research Funds for the Central Universities, the Innovative Project for Graduate Students of Jiangsu Province(No.KYLX_0100), the Scientific Research Foundation of Graduate School of Southeast University(No.YBJJ1540).
Citation: Yu Jingwen, Si Wei, Sha Jingjie, et al. Effect of salt gradients on DNA translocation through solid-state nanopores[J].Journal of Southeast University(English Edition), 2016, 32(3):307-311.DOI:10.3969/j.issn.1003-7985.2016.03.008.

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