[1] Zhang Xinjie, Zhu Hangjie, Liu Yao, et al. Stepped spiral microchannels for rapid blood plasma separation [J]. Journal of Southeast University (English Edition), 2023, 39 (2): 176-186. [doi:10.3969/j.issn.1003-7985.2023.02.009]

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Stepped spiral microchannels for rapid blood plasma separation()

用于快速分离血浆的阶梯型螺旋微流道

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

39

Issue:

2023 2

Page:

176-186

Research Field:

Mechanical Engineering

Publishing date:

2023-06-20

Info

Title:

Stepped spiral microchannels for rapid blood plasma separation

用于快速分离血浆的阶梯型螺旋微流道

Author(s):

Zhang Xinjie¹;  2;  Zhu Hangjie¹;  Liu Yao¹;  Gu Qiao³;  Zhang Yuhang¹Oseyemi Ayobami Elisha⁴;  Lü Fangrui¹;  Ni Zhonghua²

¹College of Mechanical and Electrical Engineering, Hohai University, Changzhou 213022, China
²School of Mechanical Engineering, Southeast University, Nanjing 211189, China
³Department of Gynecology and Obstetrics, The Third Affiliated Hospital of Soochow University, Changzhou 213003, China
⁴Department of Mechanical Engineering, York University, Toronto, ON M3J1P3, Canada

张鑫杰¹;  2 ;  朱行杰¹;  刘尧¹;  顾乔³;  张宇航¹Oseyemi Ayobami Elisha⁴;  吕芳蕊¹;  倪中华²

¹河海大学机电工程学院, 常州 213022; ²东南大学机械工程学院, 南京 211189; ³苏州大学附属第三医院妇产科, 常州 213003; ⁴Department of Mechanical Engineering, York University, Toronto, ON M3J1P3, Canada

Keywords:

microfluidics;  inertial focusing;  stepped spiral channel;  blood plasma separation;  secondary flow regulation

微流控;  惯性聚焦;  阶梯型螺旋流道;  血浆分离;  二次流调控

PACS:

TH77

DOI:

10.3969/j.issn.1003-7985.2023.02.009

Abstract:

Conventional ways of blood processing, such as centrifugation and filtration, are fairly limited by processing time, separation purity, clogging, and other factors. To solve this problem, a high-throughput, inertial microfluidic device composed of a stepped spiral channel is proposed for the separation of plasma from high-concentration blood. First, the particle-focusing characteristics of the stepped spiral channel are studied through the coupling of laminar flow and particle tracking modules in the COMSOL Multiphysics^�AE; software. Next, polystyrene beads are used to investigate the inertial focusing performances of the stepped spiral channel at different flow rates. Based on the experimental results of particle focusing, an optimized stepped spiral channel is applied in blood plasma separation from samples of different cell concentrations. It can be observed that the reject ratios of the blood cells are(99.72±0.13)% and(99.44±0.17)% with hematocrit(HCT)values of 0.9% and 2.25%, respectively, at an optimal flow rate of 1.5 mL/min. The ratios of blood cells rejected by the stepped spiral channel are(97.02±0.56)% and(92.92±1.53)% at ultrahigh HCT values of 4.5% and 9%, respectively. The experimental findings demonstrate that the stepped spiral channel can efficiently separate plasma from ultrahigh-concentration blood samples.

为解决传统血液处理方法如离心和过滤等存在处理时间、分离纯度、堵塞性等方面限制的问题, 设计一种高通量惯性微流控芯片, 该芯片由阶梯型螺旋流道组成, 可用于高浓度血浆分离.首先, 采用COMSOL Multiphysics^�AE;软件的层流和粒子追踪模块仿真研究阶梯型螺旋流道中粒子的聚焦特性.然后, 通过实验研究了不同流量下聚苯乙烯粒子在流道中的惯性聚焦行为.最后, 基于粒子聚焦实验结果, 选择了一种优化结构的阶梯型螺旋流道, 以用于不同浓度血液中血浆的分离应用.实验发现, 在最优流量1.5 mL/min时, 血细胞容积为0.9%和2.25%的血样中血细胞移除率分别达到(99.72±0.13)%和(99.44±0.17)%.当血细胞容积高达4.5%和9.0%时, 该阶梯型螺旋流道仍然具有(97.02±0.56)%和(92.92±1.53)%的血细胞移除率.实验结果表明, 阶梯型螺旋流道可以从高浓度血样中高效分离血浆.

References:

[1] Rafeie M, Zhang J, Asadnia M, et al. Multiplexing slanted spiral microchannels for ultra-fast blood plasma separation[J]. Lab on a Chip, 2016, 16(15): 2791-2802. DOI: 10.1039/C6LC00713A.
[2] Lee M G, Shin J H, Choi S, et al. Enhanced blood plasma separation by modulation of inertial lift force[J].Sensors and Actuators B: Chemical, 2014, 190: 311-317. DOI: 10.1016/j.snb.2013.08.092.
[3] Farahinia A, Zhang W J, Badea I. Novel microfluidic approaches to circulating tumor cell separation and sorting of blood cells: A review[J]. Journal of Science: Advanced Materials and Devices, 2021, 6(3): 303-320. DOI: 10.1016/j.jsamd.2021.03.005.
[4] Tang W L, Zhu S, Jiang D, et al. Channel innovations for inertial microfluidics[J].Lab on a Chip, 2020, 20(19): 3485-3502. DOI: 10.1039/d0lc00714e.
[5] Zhang X J, Zhu Z X, Xiang N, et al. Automated microfluidic instrument for label-free and high-throughput cell separation[J].Analytical Chemistry, 2018, 90(6): 4212-4220. DOI: 10.1021/acs.analchem.8b00539.
[6] Xiang N, Ni Z H. High-throughput concentration of rare malignant tumor cells from large-volume effusions by multistage inertial microfluidics[J].Lab on a Chip, 2022, 22(4): 757-767. DOI: 10.1039/d1lc00944c.
[7] Segré G, Silberberg A. Radial particle displacements in poiseuille flow of suspensions[J]. Nature, 1961, 189(4760): 209-210. DOI: 10.1038/189209a0.
[8] Moloudi R, Oh S, Yang C, et al. Inertial particle focusing dynamics in a trapezoidal straight microchannel: Application to particle filtration[J]. Microfluidics and Nanofluidics, 2018, 22(3): 33. DOI: 10.1007/s10404-018-2045-5.
[9] Wang X, Zandi M, Ho C C, et al. Single stream inertial focusing in a straight microchannel[J]. Lab on a Chip, 2015, 15(8): 1812-1821. DOI: 10.1039/c4lc01462f.
[10] Liu C, Hu G Q, Jiang X Y, et al. Inertial focusing of spherical particles in rectangular microchannels over a wide range of Reynolds numbers[J].Lab on a Chip, 2015, 15(4): 1168-1177. DOI: 10.1039/C4LC01216J.
[11] Hur S C, Henderson-MacLennan N K, McCabe E R B, et al. Deformability-based cell classification and enrichment using inertial microfluidics[J]. Lab on a Chip, 2011, 11(5): 912-920. DOI: 10.1039/C0LC00595A.
[12] Hur S C, Choi S E, Kwon S, et al. Inertial focusing of non-spherical microparticles[J]. Applied Physics Letters, 2011, 99(4): 044101. DOI: 10.1063/1.3608115.
[13] Yuan D, Zhang J, Sluyter R, et al. Continuous plasma extraction under viscoelastic fluid in a straight channel with asymmetrical expansion-contraction cavity arrays[J]. Lab on a Chip, 2016, 16(20): 3919-3928. DOI: 10.1039/C6LC00843G.
[14] Jiang D, Huang D, Zhao G T, et al. Numerical simulation of particle migration in different contraction-expansion ratio microchannels[J].Microfluidics and Nanofluidics, 2019, 23(1): 7. DOI: 10.1007/s10404-018-2176-8.
[15] Lee M G, Choi S, Kim H J, et al. Inertial blood plasma separation in a contraction-expansion array microchannel[J].Applied Physics Letters, 2011, 98(25): 253702. DOI: 10.1063/1.3601745.
[16] Bhagat A A S, Hou H W, Li L D, et al. Pinched flow coupled shear-modulated inertial microfluidics for high-throughput rare blood cell separation[J]. Lab on a Chip, 2011, 11(11): 1870-1878. DOI: 10.1039/C0LC00633E.
[17] Choi S, Karp J M, Karnik R. Cell sorting by deterministic cell rolling[J]. Lab on a Chip, 2012, 12(8): 1427-1430. DOI: 10.1039/C2LC21225K.
[18] Golden J P, Kim J S, Erickson J S, et al.Multi-wavelength microflow cytometer using groove-generated sheath flow[J]. Lab on a Chip, 2009, 9(13): 1942-1950. DOI: 10.1039/B822442K.
[19] Stroock A D, Dertinger S K W, Ajdari A, et al. Chaotic mixer for microchannels[J]. Science, 2002, 295(5555): 647-651. DOI: 10.1126/science.1066238.
[20] Stoecklein D, Wu C Y, Kim D, et al. Optimization of micropillar sequences for fluid flow sculpting[J].Physics of Fluids, 2016, 28(1): 012003.
[21] Amini H, Sollier E, Masaeli M, et al. Engineering fluid flow using sequenced microstructures[J]. Nature Communications, 2013, 4: 1826. DOI: 10.1038/ncomms2841.
[22] Paulsen K S, Chung A J. Non-spherical particle generation from 4D optofluidic fabrication[J].Lab on a Chip, 2016, 16(16): 2987-2995. DOI: 10.1039/C6LC00208K.
[23] Hou H W, Warkiani M E, Khoo B L, et al. Isolation and retrieval of circulating tumor cells using centrifugal forces[J]. Scientific Reports, 2013, 3: 1259. DOI: 10.1038/srep01259.
[24] Zhu Z X, Wu D, Li S, et al. A polymer-film inertial microfluidic sorter fabricated by jigsaw puzzle method for precise size-based cell separation[J].Analytica Chimica Acta, 2021, 1143: 306-314. DOI: 10.1016/j.aca.2020.11.001.
[25] Di Carlo D, Irimia D, Tompkins R G, et al. Continuous inertial focusing, ordering, and separation of particles in microchannels[J].Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(48): 18892-18897. DOI: 10.1073/pnas.0704958104.
[26] Zhang Y, Zhang J, Tang F, et al. Design of a single-layer microchannel for continuous sheathless single-stream particle inertial focusing[J]. Analytical Chemistry, 2018, 90(3): 1786-1794. DOI: 10.1021/acs.analchem.7b03756.
[27] Zhang J, Li W H, Li M, et al. Particle inertial focusing and its mechanism in a serpentine microchannel[J].Microfluidics and Nanofluidics, 2014, 17(2): 305-316. DOI: 10.1007/s10404-013-1306-6.
[28] Geng Z X, Ju Y R, Wang W, et al. Continuous blood separation utilizing spiral filtration microchannel with gradually varied width and micro-pillar array[J]. Sensors and Actuators B: Chemical, 2013, 180: 122-129. DOI: 10.1016/j.snb.2012.06.064.
[29] Shen S F, Tian C, Li T B, et al. Spiral microchannel with ordered micro-obstacles for continuous and highly-efficient particle separation[J].Lab on a Chip, 2017, 17(21): 3578-3591. DOI: 10.1039/C7LC00691H.
[30] Shen S F, Zhang F J, Wang S T, et al. Ultra-low aspect ratio spiral microchannel with ordered micro-bars for flow-rate insensitive blood plasma extraction[J].Sensors and Actuators B: Chemical, 2019, 287: 320-328. DOI: 10.1016/j.snb.2019.02.066.
[31] Warkiani M E, Guan G F, Luan K B, et al. Slanted spiral microfluidics for the ultra-fast, label-free isolation of circulating tumor cells[J]. Lab on a Chip, 2014, 14(1): 128-137. DOI: 10.1039/C3LC50617G.
[32] Kim J, Lee J, Wu C, et al. Inertial focusing in non-rectangular cross-section microchannels and manipulation of accessible focusing positions[J].Lab on a Chip, 2016, 16(6): 992-1001. DOI: 10.1039/C5LC01100K.
[33] Chen Z Z, Zhao L, Wei L J, et al. River meander-inspired cross-section in 3D-printed helical microchannels for inertial focusing and enrichment[J]. Sensors and Actuators B: Chemical, 2019, 301: 127125. DOI: 10.1016/j.snb.2019.127125.
[34] Mehran A, Rostami P, Saidi M S, et al. High-throughput, label-free isolation of white blood cells from whole blood using parallel spiral microchannels with U-shaped cross-section[J]. Biosensors, 2021, 11(11): 406. DOI: 10.3390/bios11110406.
[35] Rafeie M, Hosseinzadeh S, Taylor R A, et al. New insights into the physics of inertial microfluidics in curved microchannels. Ⅰ. Relaxing the fixed inflection point assumption[J]. Biomicrofluidics, 2019, 13(3): 034117. DOI: 10.1063/1.5109004.
[36] Zhang J, Yan S, Li W H, et al. High throughput extraction of plasma using a secondary flow-aided inertial microfluidic device[J].RSC Advances, 2014, 4(63): 33149-33159. DOI: 10.1039/C4RA06513A.
[37] Xiang N, Ni Z H. High-throughput blood cell focusing and plasma isolation using spiral inertial microfluidic devices[J].Biomedical Microdevices, 2015, 17(6): 110. DOI: 10.1007/s10544-015-0018-y.
[38] Mach A J, Di Carlo D. Continuous scalable blood filtration device using inertial microfluidics[J].Biotechnology and Bioengineering, 2010, 107(2): 302-311. DOI: 10.1002/bit.22833.
[39] Robinson M, Marks H, Hinsdale T, et al. Rapid isolation of blood plasma using a cascaded inertial microfluidic device[J].Biomicrofluidics, 2017, 11(2): 024109. DOI: 10.1063/1.4979198.
[40] Han J Y, DeVoe D L. Plasma isolation in a syringe by conformal integration of inertial microfluidics[J]. Annals of Biomedical Engineering, 2021, 49(1): 139-148. DOI: 10.1007/s10439-020-02526-9.
[41] Di Carlo D. Inertial microfluidics[J]. Lab on a Chip, 2009, 9(21): 3038-3046.
[42] Kuntaegowdanahalli S S, Bhagat A A S, Kumar G, et al. Inertial microfluidics for continuous particle separation in spiral microchannels[J]. Lab on a Chip, 2009, 9(20): 2973-2980. DOI: 10.1039/B908271A.[LinkOut]
[43] Han S, Zhang X J, Gu Q, et al. Inertial focusing effect of particles in spiral microchannel with asymmetric cross-section[J]. Optics and Precision Engineering, 2022, 30(3): 310-319. DOI:10.37188/OPE.20223003.0310. (in Chinese)

Memo

Memo:

Biography: Zhang Xinjie(1984—), male, doctor, associate professor, xj.zhang@hhu.edu.cn.
Foundation items: The National Natural Science Foundation of China(No.51905150), Fundamental Research Funds for the Central Universities(No.B220202024), Changzhou Science and Technology Bureau Program(No.CE20225046), Changzhou Health Commission Youth Science and Technology Projects(No.QN202115), Jiangsu Planned Projects for Postdoctoral Research Funds(No.2019K033), Postgraduate Research and Practice Innovation Program of Jiangsu Province(No.KYCX23_0670).
Citation: Zhang Xinjie, Zhu Hangjie, Liu Yao, et al. Stepped spiral microchannels for rapid blood plasma separation[J].Journal of Southeast University(English Edition), 2023, 39(2):176-186.DOI:10.3969/j.issn.1003-7985.2023.02.009.

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