[1] Zhang Yuanying, Guo Huishi, He Nongyue, et al. Applications of gene-chip for the detection of mutationsin cTnI gene associated with FHCM [J]. Journal of Southeast University (English Edition), 2005, 21 (3): 330-335. [doi:10.3969/j.issn.1003-7985.2005.03.017]

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Applications of gene-chip for the detection of mutationsin cTnI gene associated with FHCM()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

21

Issue:

2005 3

Page:

330-335

Research Field:

Biological Science and Medical Engineering

Publishing date:

2005-09-30

Info

Title:

Applications of gene-chip for the detection of mutationsin cTnI gene associated with FHCM

Author(s):

Zhang Yuanying¹;  3;  Guo Huishi¹;  He Nongyue¹;  2;  Gu Yu¹;  Yang Di²;  Wan Wenhui²;  Zhang Jinan²

¹ State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
² Research Institute of Cardiovascular Disease, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
³Jiangsu Institute of Cancer Research, Nanjing 210009, China

Keywords:

hypertrophic cardiomyopathy(HCM);  gene-chip;  mutation;  hybridization

PACS:

Q81

DOI:

10.3969/j.issn.1003-7985.2005.03.017

Abstract:

Mutations in cardiac troponin I(cTnI)gene were assessed based on gene-chip technology.Special probes were designed to fabricate the low-density gene-chip, which could detect the mutations in exons 3, 5, 7, and 8 of the cTnI gene simultaneously.For each exon, two oligonucleotide sequences labeled with fluorescein at the 5′-end were designed, one(oligonucleotide Ⅰ)simulating the wild type and the other(oligonucleotide Ⅱ)simulating the mutant.Oligonucleotides Ⅰ and Ⅱ were mixed together to simulate the heterozygote.After optimizing the hybridization protocols, the fabricated gene-chip could detect the mutations in the exons of the cTnI gene with relative high sensitivity and specificity.The fully complementary probe gave a fluorescent signal almost 50% stronger than that of the one-base mismatched one, which is in accordance with the result from a theoretical estimate. An applicable special gene-chip is available to investigate and diagnose familial hypertrophic cardiomyopathy(FHCM)after further improvement.

References:

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[7] Thierfelder L, MacRae C, Watkins H, et al.A familial hypertrophic cardiomyopathy locus maps to chromosome 15q2 [J].Proc Natl Acad Sci USA, 1993, 90(13):6270-6274.
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Memo

Memo:

Biographies: Zhang Yuanying(1978—), female, master;He Nongyue(corresponding author), male, doctor, professor, nyhe1958@163.com.

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