[1] Zhang Ling, Tan Jiajun, Shi Xiaolei, et al. Fabrication IL-1Ra loaded galactosylated chitosan nanoparticlesfor liver targeting [J]. Journal of Southeast University (English Edition), 2012, 28 (4): 469-473. [doi:10.3969/j.issn.1003-7985.2012.04.017]

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Fabrication IL-1Ra loaded galactosylated chitosan nanoparticlesfor liver targeting()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

28

Issue:

2012 4

Page:

469-473

Research Field:

Chemistry and Chemical Engineering

Publishing date:

2012-12-30

Info

Title:

Fabrication IL-1Ra loaded galactosylated chitosan nanoparticlesfor liver targeting

Author(s):

Zhang Ling¹;  2;  Tan Jiajun³;  Shi Xiaolei ³;  Xu Shi¹;  2;  Xu Qian¹;  2

¹Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, Southeast University, Nanjing 210009, China
²State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
³

Keywords:

nanoparticle;  galactosylated chitosan;  electrospraying;  liver targeting

PACS:

TQ31

DOI:

10.3969/j.issn.1003-7985.2012.04.017

Abstract:

Galactosylated chitosan(GC)is synthesized and used to prepare IL-1Ra loaded GC nanoparticles by an electrospraying technique. Polyethylene oxide(PEO)is mixed with GC to enhance the electrospraying ability. The effect of the spraying solution properties on particle formation is investigated. The IL-1Ra loaded nanoparticles with an average diameter of 530 nm and a regularly spherical shape are observed by the scanning electron microscopy(SEM). The amount of the IL-1Ra is measured by the enzyme-linked immunosorbent assay(ELISA)kit. The loading capacity of the nanoparticle is(1.52±0.04)%(n=3)and the encapsulation efficiency reaches(90.36±3.46)%(n=3). For the evaluation of GC nanoparticles’ hepatocytes targeting efficacy, hepatocytes and mesenchymal stem cells(MSCs)are incubated with FITC-labeled GC nanoparticles for 24 h as the experimental and control groups. Results of the fluorescence microscope show that the fluorescence signals observed in hepatocytes are significantly higher than in the MSCs, indicating that the developed GC nanoparticles have an obvious liver targeting property.

References:

[1] Vila A, Sánchez A, Tobío M, et al. Design of biodegradable particles for protein delivery [J]. Journal of Controlled Release, 2002, 78(1/2/3):15-24.
[2] Xie J W, Ng W J, Lee L Y, et al. Encapsulation of protein drugs in biodegradable microparticles by co-axial electrospray [J]. Journal of Colloid and Interface Science, 2008, 317(2):469-476.
[3] Santander-Ortega M J, Csaba N, González L, et al. Protein-loaded PLGA—PEO blend nanoparticles: encapsulation, release and degradation characteristics [J].Colloid Polymer Science, 2010, 288(2):141-150.
[4] Xie J W, Wang C H. Encapsulation of proteins in biodegradable polymeric microparticles using electrospray in the Taylor cone-jet mode [J]. Biotechnology and Bioengineering, 2007, 97(5):1278-1290.
[5] Scholten E, Dhamankar H, Bromberg L. Electrospray as a tool for drug micro-and nanoparticle patterning [J]. Langmuir, 2011, 27(11):6683-6688.
[6] Chakraborty S, Liao I C, Adler A, et al. Electrohydrodynamics: a facile technique to fabricate drug delivery system [J]. Advanced Drug Delivery Reviews, 2009, 12(61):1043-1054.
[7] Ji W, Yang F, Zhuan B. Fibrous scaffolds loaded with protein prepared by blend or coaxial electrospinning [J]. Acta Biomaterialia, 2010, 6(11):4199-4207.
[8] Shinoda M, Tilles A W, Kobayashi N, et al. A bioartificial liver device secreting interleukin-1 receptor antagonist for the treatment of hepatic failure in rat [J].Journal of Surgical Research, 2007, 137(1):130-140.
[9] Liang H F, Chen C T, Chen S C et al. Paclitaxel-loaded poly(g-glutamic acid)-poly(lactide)nanoparticles as a targeted drug delivery system for the treatment of liver cancer [J]. Biomaterials, 2006, 27(9): 2051-2059.
[10] Arya N, Chakraborty S, Dube N, et al. Electrospraying: a facile technique for synthesis of chitosan-based micro/nanospheres for drug delivery applications [J]. Journal of Biomedical Materials Research, 2009, 88(1):17-31.
[11] Wang Q, Zhang L, Hu W, et al. Norcantharidin-associated galactosylated chitosan nanoparticles for hepatocyte-targeted delivery [J]. Nanomedicine: Nanotechnology, Biology and Medicine, 2010, 6(2):371-381.
[12] Narayan B, Dennis E, Omid V, et al. Electrospun chitosan-based nanofibers and their cellularcompatibility [J]. Biomaterials, 2005, 26(31):6176-6184.
[13] Feng Z Q, Chu X H, Huang N P, et al. The effect of nanofibrous galactosylated chitosan scaffolds on the formation of rat primary hepatocyte aggregates and the maintenance of liver function [J]. Biomaterials, 2009, 14(30):2753-2763.
[14] Mi F L, Wu Y L, Chiu Y L, et al. Synthesis of a novel glycoconjugated chitosan and preparation of its derived nanoparticles for targeting HepG2 cells [J]. Biomacromolecules, 2007, 8(3):892-898.
[15] Magalhães R, Nugraha B, Pervaiz S, et al. Influence of cell culture configuration on the post-cryopreservation viability of primary rat hepatocytes [J]. Biomaterials, 2012, 33(3):829-836.
[16] Olde Damink L H H, Dijkstra P J, van Luyn M J A, et al. In vitro degradation of dermal sheep collagen cross-linked using a water soluble carbodiimide [J]. Biomaterials, 1996, 17(7):679-684.
[17] Zhang S L, Kawakami K. One-step preparation of chitosan solid nanoparticles by electrospray deposition [J]. International Journal of Pharmaceutics, 2010, 397(1/2):211-217.

Memo

Memo:

Biographies: Zhang Ling(1987—), female, graduate; Xu Qian(corresponding author), female, doctor, associate professor, q_-xu@163.com.
Foundation items: The National Natural Science Foundation of China(No.30901431), the Natural Science Foundation of Jiangsu Province(No.BK2010242).
Citation: Zhang Ling, Tan Jiajun, Shi Xiaolei, et al. Fabrication IL-1Ra loaded galactosylated chitosan nanoparticles for liver targeting[J].Journal of Southeast University(English Edition), 2012, 28(4):469-473.[doi:10.3969/j.issn.1003-7985.2012.04.017]

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