[1] Wang Ziyu, Zhang Dongsheng, Gu Ning, Lu Xinli, et al. Preparation and evaluation of As2O3 nanoparticlesfor treatment of human liver cancer cells in vitro [J]. Journal of Southeast University (English Edition), 2005, 21 (1): 58-62. [doi:10.3969/j.issn.1003-7985.2005.01.013]

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Preparation and evaluation of As₂O₃ nanoparticlesfor treatment of human liver cancer cells in vitro()

As₂O₃ 纳米粒的制备、表征和体外治疗人肝癌细胞的研究

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

21

Issue:

2005 1

Page:

58-62

Research Field:

Biological Science and Medical Engineering

Publishing date:

2005-03-30

Info

Title:

Preparation and evaluation of As₂O₃ nanoparticlesfor treatment of human liver cancer cells in vitro

As₂O₃ 纳米粒的制备、表征和体外治疗人肝癌细胞的研究

Author(s):

Wang Ziyu¹, Zhang Dongsheng¹, Gu Ning², Lu Xinli¹, Yan Shiyan¹

¹School of Basic Medical Science, Southeast University, Nanjing 210009, China
²Department of Biomedical Science and Engineering, Southeast University, Nanjing 210096, China

王子妤¹, 张东生¹, 顾宁², 路新丽¹, 颜士岩¹

¹东南大学基础医学院, 南京 210009; ²东南大学生物科学与医学工程系, 南京 210096

Keywords:

nanotechnology;  arsenic trioxide;  liver cancer

纳米技术;  As₂O₃;  肝癌

PACS:

R73-36

DOI:

10.3969/j.issn.1003-7985.2005.01.013

Abstract:

This paper studies the preparation method of As₂O₃ nanoparticles and their antitumor effect on human liver cancer cells.As₂O₃ nanoparticles were prepared by sol-gel method.As₂O₃ nanoparticles were characterized by transmission electron microscope(TEM), energy dispersive spectrometer(EDS)and computer color magic image analysis system(CMIAS).A methyl thiazolyl tetrazolium(MTT)assay and a flow cytometry(FCM)assay were performed to examine the antitumor effect of As₂O₃ nanoparticles at various concentrations(1, 2, 5, 10 μmol/L).We also compared the antitumor effect of As₂O₃ nanoparticles with that of As₂O₃ solution.The average diameters of two kinds of As₂O₃ nanoparticles prepared were about 80 nm and 40 nm.It was identified that the prepared nanoparticles were As₂O₃ and there were no other components by EDS.After 48 h of treatment with As₂O₃ nanoparticles, the survival ratio of cells was significantly lower than that of the As₂O₃ solution with the same concentration(P<0.05).Experimental results demonstrate that by sol-gel method As₂O₃ can be prepared into nanoparticles.As₂O₃ nanoparticles can produce a better cytotoxic effect on tumor cells than the As₂O₃ solution.

研究了As₂O₃ 纳米粒的制备方法、表征及其抗肝癌细胞的作用.采用溶胶-凝胶法制备As₂O₃ 纳米粒, 并用透射电镜、能谱仪和图像分析仪等方法对其进行表征及特性检测.通过MTT法和流式细胞仪法研究了不同浓度As₂O₃ 纳米粒对人肝癌细胞株的影响, 并与传统的As₂O₃ 溶液进行了比较.实验中制备了2种粒径大小的As₂O₃ 纳米粒子, 其平均直径分别为80 nm和40 nm, 通过能谱仪的测试证实所制备的纳米粒为As₂O₃, 且无其他成分.体外细胞实验发现, As₂O₃ 纳米粒处理细胞48 h后, 人肝癌细胞的存活率明显低于同浓度的As₂O₃ 溶液处理组(P<0.05).研究结果显示, 通过溶胶-凝胶法可将As₂O₃ 制备成纳米粒子;体外细胞实验表明, 与传统的As₂O₃ 溶液相比, As₂O₃纳米粒子可对肿瘤细胞产生更强细胞毒作用.

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Memo

Memo:

Biographies: Wang Ziyu(1979—), female, graduate;Zhang Dongsheng(corresponding author), male, professor, b7712900@jlonline.com.

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