[1] Duan Yanbing, Yu Jia, Liu Haifeng, et al. Determination of anthraquinone prodrugand its hydrolytically active compounds using RP-HPLC [J]. Journal of Southeast University (English Edition), 2009, 25 (1): 128-131. [doi:10.3969/j.issn.1003-7985.2009.01.027]

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Determination of anthraquinone prodrugand its hydrolytically active compounds using RP-HPLC()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

25

Issue:

2009 1

Page:

128-131

Research Field:

Biological Science and Medical Engineering

Publishing date:

2009-03-30

Info

Title:

Determination of anthraquinone prodrugand its hydrolytically active compounds using RP-HPLC

Author(s):

Duan Yanbing¹;  2;  Yu Jia³;  Liu Haifeng¹;  2;  Ji Min¹;  2

¹School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210096, China
²Key Laboratory of Biomaterial and Technology, Suzhou Research Institute of Southeast University, Suzhou 215123, China
³College of Chengxian, Southeast University, Nanjing 210088, China

Keywords:

high-performance liquid chromatography(HPLC);  quantitative analysis;  anthraquinone prodrug;  active components

PACS:

R917

DOI:

10.3969/j.issn.1003-7985.2009.01.027

Abstract:

In order to study the hydrolytic characterization of an anti-inflammatory prodrug(RI-1)in vitro, an effective, accurate and reliable method for the simultaneous determination of the prodrug and its two hydrolytic active compounds is developed using reverse phase high-performance liquid chromatography(RP-HPLC).The chromatographic separation is performed on an ODS-2 C₁₈ column(250 mm×4.6 mm, 5.0 μm particle size)with a simple elution program.The mobile phase is V(methanol)∶V(0.1%phosphoric acid solution)=90∶10(adjust pH to 2.3).A wavelength of 225 nm and a mobile phase flow rate of 1.0 mL/min are utilized for the quantitative analysis.Excellent linear behaviors over the investigated concentration ranges are observed with values of R² higher than 0.999 for all the analytes.The validated method is successfully applied to the simultaneous determination of the prodrug and its active components can be used to detect hydrolytic characterization in vitro.

References:

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Memo

Memo:

Biographies: Duan Yanbing(1978—), male, graduate;Ji Min(corresponding author), female, doctor, professor, jimin@seu.edu.cn.
Citation: Duan Yanbing, Yu Jia, Liu Haifeng, et al.Determination of anthraquinone prodrug and its hydrolytically active compounds using RP-HPLC[J].Journal of Southeast University(English Edition), 2009, 25(1):128-131.

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