[1] Zhang Niping, Yu Shuqin, Wang Hui, Shen Yanyan, et al. Method for quantification of prazosin in dog plasmaand its application to pharmacokinetic study [J]. Journal of Southeast University (English Edition), 2011, 27 (2): 217-221. [doi:10.3969/j.issn.1003-7985.2011.02.022]

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Method for quantification of prazosin in dog plasmaand its application to pharmacokinetic study()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

27

Issue:

2011 2

Page:

217-221

Research Field:

Chemistry and Chemical Engineering

Publishing date:

2011-06-30

Info

Title:

Method for quantification of prazosin in dog plasmaand its application to pharmacokinetic study

Author(s):

Zhang Niping¹;  Yu Shuqin²;  Wang Hui²;  Shen Yanyan¹;  3;  Xu Shi¹;  Zhang Ling¹;  Xu Qian¹;  3

¹ Ministry of Education Key Laboratory of Environmental Medicine and Engineering, Southeast University, Nanjing 210009, China
² Jiangsu Key Laboratory for Medical Supermolecule Material and Application, Nanjing Normal University, Nanjing 210046, China
³ Suzhou Key Laboratory of Environment and Biosafety, Suzhou Institute, Southeast University, Suzhou 215123, China

Keywords:

prazosin(PZS);  PZS-sulfobutyl ether beta-cyclodextrin(PZS-SBE-β-CD)inclusion complex tablets;  high performance liquid chromatography(HPLC);  pharmacokinetics

PACS:

O657

DOI:

10.3969/j.issn.1003-7985.2011.02.022

Abstract:

A simple and sensitive high performance liquid chromatography method using fluorescence detection(HPLC-FLD)and a one-step single solvent extraction for the determination of prazosin(PZS)in dog plasma is developed and validated. After extraction with ether, the chromatographic separation of PZS is carried out using a reverse phase C18 column(150 mm×4.6 mm, 5 μm)with a mobile phase of 30% acetonitrile and 70% acetic acid-sodium acetate buffer solution(pH=3.6)and quantified by fluorescence detection operated with an excitation wavelength of 258 nm and an emission wavelength of 387 nm. The flow rate of the mobile phase is 1.0 mL/min and the retention time of PZS and the internal standard is found to be 4.4 and 5.8 min, respectively. The calibration curve is linear within a concentration range from 1.0 to 1 000.0 ng/mL(r²>0.998). The limit of detection is 0.4 ng/mL. The inter-day coefficient of variation(COV)of the calibration standards is below 5.0% and the mean accuracy is in the range from 92.7% to 104.2%. Moreover, by analyzing quality control plasma samples for three days, the results show that the method is precise and accurate, for the intra- and inter-day COV within 10% and the accuracy from 95.9% to 112.7%. The developed and validated method is successfully applied to pharmacokinetic study for the preclinical evaluation of a new peroral PZS-sulfobutyl ether beta-cyclodextrin(PZS-SBE-β-CD)inclusion complex tablets(test preparation), which demonstrates that the test preparation released PZS is conducted in a slow and controlled way, and the relative bioavailability of the test preparation is found to be 105.0%.

References:

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Memo

Memo:

Biographies: Zhang Niping(1987—), female, graduate; Xu Qian(corresponding author), female, doctor, associate professor, q_xu@163.com.
Foundation items: Pre-Research Foundation for the National Natural Science Foundation of Southeast University(No. 9225000007), Suzhou Science and Technology Development Projects(No.YJS0948).
Citation: Zhang Niping, Yu Shuqin, Wang Hui, et al. Method for quantification of prazosin in dog plasma and its application to pharmacokinetic study[J].Journal of Southeast University(English Edition), 2011, 27(2):217-221.[doi:10.3969/j.issn.1003-7985.2011.02.022]

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