[1] Meng Junli, Wu Min, Ding Wen, Li Ying, et al. Metal cation crosslinking of TiO2-alginate hybrid gels [J]. Journal of Southeast University (English Edition), 2014, 30 (4): 526-530. [doi:10.3969/j.issn.1003-7985.2014.04.021]

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Metal cation crosslinking of TiO₂-alginate hybrid gels()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

30

Issue:

2014 4

Page:

526-530

Research Field:

Biological Science and Medical Engineering

Publishing date:

2014-12-31

Info

Title:

Metal cation crosslinking of TiO₂-alginate hybrid gels

Author(s):

Meng Junli;  Wu Min;  Ding Wen;  Li Ying;  Zhang Jin;  Ni Henmei

School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China

Keywords:

cationic crosslinker;  diffusion;  hybrid gel;  ionotropic gelation;  Michaelis-Menten constant;  titanium dioxide modified alginate

PACS:

Q814.2

DOI:

10.3969/j.issn.1003-7985.2014.04.021

Abstract:

In order to improve the substrate diffusion properties and stability of an immobilized enzyme, alginate microgels modified with TiO₂ nanoparticles were employed as the enzyme immobilizing support. Ionotropic gelation was applied for the preparation of hybrid gels, while Ca²⁺, Ce³⁺, Ni²⁺, Cu²⁺ and Fe³⁺ were employed as the crosslinkers. Papain was selected as the model enzyme. UV-Vis spectroscopy was employed to investigate the activity of papain to evaluate kinetics and stability. Analysis results show that the highest affinity, the lowest Michaelis-Menten constant(K_m=11.0 mg/mL)and the highest stability are obtained when using Cu²⁺ as the crosslinker. The effect of the mass ratio of TiO₂ to papain on the stability and leakage of papain is also investigated, and the results show that 10∶1(TiO₂∶papain)is optimal because the proper use of TiO₂ can reduce enzyme leakage and ensure enzyme stability. Preparing Cu/alginate/TiO₂ hybrid gels via ionotropic gelation can provide a satisfactory diffusion capability and enzyme stability.

References:

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Memo

Memo:

Biographies: Meng Junli(1986—), female, graduate; Wu Min(corresponding author), female, doctor, professor, seuwumin@seu.edu.cn.
Foundation items: The National Natural Science Foundation of China(No.21005016), the Foundation of Educational Commission of Jiangsu Province(No.JHB2011-2).
Citation: Meng Junli, Wu Min, Ding Wen, et al. Metal cation crosslinking of TiO₂-alginate hybrid gels[J].Journal of Southeast University(English Edition), 2014, 30(4):526-530.[doi:10.3969/j.issn.1003-7985.2014.04.021]

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