[1] Zhang Zhi, Liu Dongmin, Xu Bingxin, Hu Junfei, et al. Liquid-state fermentation with Bacillus subtilis Bs-07 to enhanceanticoagulant function of Auricularia auricula polysaccharide [J]. Journal of Southeast University (English Edition), 2015, 31 (1): 143-148. [doi:10.3969/j.issn.1003-7985.2015.01.024]

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Liquid-state fermentation with Bacillus subtilis Bs-07 to enhanceanticoagulant function of Auricularia auricula polysaccharide()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

31

Issue:

2015 1

Page:

143-148

Research Field:

Publishing date:

2015-03-30

Info

Title:

Liquid-state fermentation with Bacillus subtilis Bs-07 to enhanceanticoagulant function of Auricularia auricula polysaccharide

Author(s):

Zhang Zhi¹;  Liu Dongmin¹;  Xu Bingxin¹;  Hu Junfei¹;  Liu Qi¹Guo Yong¹;  Liang Zhuoran¹;  Lu Xiangni¹;  Zhao Zichen¹;  Wang Zhenyu²

¹College of Forestry, Northeast Forestry University, Harbin 150040, China
²School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China

Keywords:

Auricularia auricula polysaccharide;  microbial fermentation;  anticoagulation

PACS:

中图分类号:TS202.3

DOI:

10.3969/j.issn.1003-7985.2015.01.024

Abstract:

In order to improve the anticoagulant function of Auricularia auricula, Auricularia auricula polysaccharide(AAP)was converted into its derivatives by the microbial fermentation method and then polysaccharide derivatives with stronger anticoagulant activity were prepared. The optimal conditions for fermenting the polysaccharide from A. auricula were examined and the in vitro anticoagulant activities of transformed and untransformed polysaccharides were compared. Response surface tests and an orthogonal experiment indicated that the best conditions for microbial conversion of AAP₃ were an AAP₃ concentration of 4.0 mg/mL, a ratio of substrate(AAP₃)to donor(p-hydroxybenzoic acid)of 40∶1, and a pH of 6.0. Bacillus subtilis Bs-07 was inoculated and then placed on a rotary shaker(120 r/min), followed by fermentation for 48 h at 35 ℃.The conversion rate was found to be greater than 40%. The result of in vitro anticoagulant activity showed that the transformed polysaccharide improved activated partial thromboplastin time, prothrombin time, and thrombin time values and greatly enhanced anticoagulant activity compared to the untransformed polysaccharide.

References:

[1] Zhang Zhi. Researches in selecting acylated strains of Lonicera edulis Turcz, derivatives structure and function [D]. Harbin: Northeast Forestry University, 2011.(in Chinese)
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Memo

Memo:

Biographies: Zhang Zhi(1964—), female, doctor, professor, Ldzhangzhi@163.com; Wang Zhenyu(corresponding author), male, doctor, professor, wzy219001@163.com.
Foundation items: Heilongjiang Province Application Technology Research and Development Plan(No. GA13B202), Heilongjiang Postdoctoral Foundation(No. LBH-Z13008).
Citation: Zhang Zhi, Liu Dongmin, Xu Bingxin, et al. Liquid-state fermentation with Bacillus subtilis Bs-07 to enhance anticoagulant function of Auricularia auricula polysaccharide[J].Journal of Southeast University(English Edition), 2015, 31(1):143-148.[doi:10.3969/j.issn.1003-7985.2015.01.024]

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