[1] Zhang Zhi, Liu Dongmin, Xu Bingxin, Hu Junfei, et al. Liquid-state fermentation with Bacillus subtilis Bs-07 to enhanceanticoagulant function of Auricularia auricula polysaccharide [J]. Journal of Southeast University (English Edition), 2015, 31 (1): 143-148. [doi:10.3969/j.issn.1003-7985.2015.01.024]

Copy

Liquid-state fermentation with Bacillus subtilis Bs-07 to enhanceanticoagulant function of Auricularia auricula polysaccharide()

枯草芽孢杆菌Bs-07液态发酵提高黑木耳多糖抗凝血功能

Share：

Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

31

Issue:

2015 1

Page:

143-148

Research Field:

Publishing date:

2015-03-30

Info

Title:

Liquid-state fermentation with Bacillus subtilis Bs-07 to enhanceanticoagulant function of Auricularia auricula polysaccharide

枯草芽孢杆菌Bs-07液态发酵提高黑木耳多糖抗凝血功能

Author(s):

Zhang Zhi¹;  Liu Dongmin¹;  Xu Bingxin¹;  Hu Junfei¹;  Liu Qi¹Guo Yong¹;  Liang Zhuoran¹;  Lu Xiangni¹;  Zhao Zichen¹;  Wang Zhenyu²

¹College of Forestry, Northeast Forestry University, Harbin 150040, China
²School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China

张智¹;  刘东敏¹;  徐冰心¹;  胡俊飞¹;  刘奇¹国勇¹;  梁卓然¹;  卢香泥¹;  赵梓辰¹;  王振宇²

¹东北林业大学林学院, 哈尔滨150040; ²哈尔滨工业大学食品科学与工程学院, 哈尔滨150090

Keywords:

Auricularia auricula polysaccharide;  microbial fermentation;  anticoagulation

黑木耳多糖;  微生物发酵;  抗凝血

PACS:

中图分类号:TS202.3

DOI:

10.3969/j.issn.1003-7985.2015.01.024

Abstract:

In order to improve the anticoagulant function of Auricularia auricula, Auricularia auricula polysaccharide(AAP)was converted into its derivatives by the microbial fermentation method and then polysaccharide derivatives with stronger anticoagulant activity were prepared. The optimal conditions for fermenting the polysaccharide from A. auricula were examined and the in vitro anticoagulant activities of transformed and untransformed polysaccharides were compared. Response surface tests and an orthogonal experiment indicated that the best conditions for microbial conversion of AAP₃ were an AAP₃ concentration of 4.0 mg/mL, a ratio of substrate(AAP₃)to donor(p-hydroxybenzoic acid)of 40∶1, and a pH of 6.0. Bacillus subtilis Bs-07 was inoculated and then placed on a rotary shaker(120 r/min), followed by fermentation for 48 h at 35 ℃.The conversion rate was found to be greater than 40%. The result of in vitro anticoagulant activity showed that the transformed polysaccharide improved activated partial thromboplastin time, prothrombin time, and thrombin time values and greatly enhanced anticoagulant activity compared to the untransformed polysaccharide.

为了提高黑木耳多糖的抗凝血功能, 应用微生物发酵技术转化黑木耳多糖得到抗凝血活性更强的黑木耳多糖衍生物.研究了黑木耳多糖转化的最佳发酵条件, 比较了未转化和转化后的黑木耳多糖的体外抗凝血功能.通过响应面和正交试验得出最佳转化条件:将浓度4.0 mg/mL的黑木耳酸性多糖AAP₃溶液与对羟基苯甲酸充分混合(体积比为40〓 ∶1), 调pH至6.0, 接种枯草芽孢杆菌Bs-07, 在温度为35 ℃, 旋转摇床转速为120 r/min的条件下, 发酵48 h, 转化率达40%以上.检测转化后黑木耳多糖衍生物APTT, PT和TT的数据结果显示, 转化后黑木耳多糖衍生物体外抗凝血功能得到了明显提高.

References:

[1] Zhang Zhi. Researches in selecting acylated strains of Lonicera edulis Turcz, derivatives structure and function [D]. Harbin: Northeast Forestry University, 2011.(in Chinese)
[2] Lai Ping, Lin Yuexin. Developments in molecular modification of natural polysaccharides [J]. Chemistry of Life, 2003, 23(3): 183-187.(in Chinese)
[3] Zhang Hua, Wang Zhenyu, Zhang Zhi. In vitro antioxidant activities of sulfated derivatives of polysaccharides extracted from Auricularia auricular[J]. International Journal of Molecular Sciences, 2011, 12(5):3288-3302.
[4] Ye Tingmei, Qian Lingbo, Cui Jie, et al. Auricularia auricular polysaccharide protects myocardium against ischemia/reperfusion injury [J]. Chinese Journal of Applied Physiology, 2010, 26(2):154-158.(in Chinese)
[5] Li Ling, Ding Junnan, Luo Li, et al. Preliminary studies on the early quality identification of Auricularia auricular [J]. Journal of Forestry Research, 2005, 16(1):61-64.
[6] Chen Hesheng, Sun Zhenya. Studies on polysaccharides of Auricularia auricularjuade [J]. Lishizhen Medicine and Materia Medica Research, 2003, 14(5):300-301.(in Chinese)
[7] Li Gongbin. Studies on molecular modification function as well as application of Auricularia auricula polysaccharides [D]. Harbin: Northeast Forestry University, 2006.(in Chinese)
[8] Fan Lisheng. Studies on preparation and bioactivities of the AAP-IIa fraction of polysaccharides from Auricularia auricula [D]. Wuhan: Huazhong Agricultural University, 2006.(in Chinese)
[9] Ouyang Tianzhi, Xie Jiugao. Isolation, purification and characterization of Alkali-soluble polysaccharides of “KIKURAGE, ” fruit body of Auricularia auriada-judae [J]. Journal of Huazhong Agricultural University, 1999, 18(1): 46-48.(in Chinese)
[10] Li J, Wei Y X, Du G C, et al. Anticoagulant activities of phlorotannins from Sargassum thunbergii Kuntze[J]. Traditional Chinese Drug Research & Clinical Pharmacology, 2007, 18(3): 191-194.
[11] Song Xiao, Zhang Lihua, Zhao Peng, et al. Researches in optimizing acetylating of Tussilago farfara polysaccharides by using response surface methodology [J]. Chinese Traditional Patent Medicine, 2013, 35(9):2030-2033.(in Chinese)
[12] Wang Yunxiang, Lu Zhao. Fermentation, bioactivity and structure of extracellular polysaccharide by clitocybe sp.AS5.112 strain [J].Journal of Nanjing Agricultural University, 2004, 27(3):94-98.(in Chinese)

Memo

Memo:

Biographies: Zhang Zhi(1964—), female, doctor, professor, Ldzhangzhi@163.com; Wang Zhenyu(corresponding author), male, doctor, professor, wzy219001@163.com.
Foundation items: Heilongjiang Province Application Technology Research and Development Plan(No. GA13B202), Heilongjiang Postdoctoral Foundation(No. LBH-Z13008).
Citation: Zhang Zhi, Liu Dongmin, Xu Bingxin, et al. Liquid-state fermentation with Bacillus subtilis Bs-07 to enhance anticoagulant function of Auricularia auricula polysaccharide[J].Journal of Southeast University(English Edition), 2015, 31(1):143-148.[doi:10.3969/j.issn.1003-7985.2015.01.024]

Common functions