[1] Li Yunhui, Zhou Jue, Zhang Minhui, Yang MingLi Xiaobo, et al. Effects of microcystin-LR on hippocampal N-acetylaspartateand neurobehaviors in rats [J]. Journal of Southeast University (English Edition), 2015, 31 (4): 577-581. [doi:10.3969/j.issn.1003-7985.2015.04.025]

Copy

Effects of microcystin-LR on hippocampal N-acetylaspartateand neurobehaviors in rats()

微囊藻毒素-LR对大鼠海马组织中 N-乙酰门冬氨酸及神经行为的作用

Share：

Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

31

Issue:

2015 4

Page:

577-581

Research Field:

Biological Science and Medical Engineering

Publishing date:

2015-12-30

Info

Title:

Effects of microcystin-LR on hippocampal N-acetylaspartateand neurobehaviors in rats

微囊藻毒素-LR对大鼠海马组织中 N-乙酰门冬氨酸及神经行为的作用

Author(s):

Li Yunhui¹;  Zhou Jue¹;  Zhang Minhui¹;  Yang Ming²Li Xiaobo¹;  Liu Ran¹;  Yin Lihong¹;  Pu Yuepu¹

¹Key Laboratory of Environmental Medicine Engineering of Ministry of Education, Southeast University, Nanjing 210009, China
²Jiangsu Key Laboratory of Molecule Imaging and Functional Imaging, Southeast University, Nanjing 210009, China

李云晖¹;  周珏¹;  张敏辉¹;  杨明²;  李晓波¹;  刘冉¹;  尹立红¹;  浦跃朴¹

¹东南大学环境医学工程教育部重点实验室, 南京 210009; ²东南大学江苏省分子影像与功能影像重点实验室, 南京 210009

Keywords:

low-doses of microcystin-leucine-arginine(MC-LR);  N-acetylaspartate(NAA);  neurobeha-viors;  magnetic resonance spectroscopy;  neuro

微囊藻毒素-LR;  N-乙酰门冬氨酸;  神经行为;  核磁共振成像;  神经元

PACS:

R12

DOI:

10.3969/j.issn.1003-7985.2015.04.025

Abstract:

The effects of low-doses of microcystin-leucine-arginine(MC-LR)exposure on neurobehaviors and N-acetylaspartate(NAA)expression in the hippocampus of rats were investigated. After male Sprague-Dawley(SD)rats were treated intra-gastrically with different doses of MC-LR for 90 d, the locomotor activity, spatial learning and memory function were evaluated in the rats after treatment using open field tests and Morris water maze tests. The results show that MC-LR exposure can lead to impairment of the spatial learning capacity and locomotor activity in rats at the dose of 2.00 μg/kg. The levels of NAA in the hippocampus were measured by magnetic resonance spectroscopy(MRI). A significant decrease of NAA/Cr ratio(P<0.05)was observed in the hippocampous. This study indicates that intra-gastrical exposure to low-doses of MC-LR has adverse effects on neuronal behavior and NAA levels in the hippocampous.

探讨了微囊藻毒素-LR(MC-LR)对大鼠神经行为及海马组织中神经递质N-乙酰门冬氨酸(NAA)水平的影响.用不同剂量的MC-LR对雄性sprague-dawley(SD)大鼠灌胃染毒90 d后, 进行旷场实验和Morris水迷宫试验来评估大鼠的运动行为、空间学习能力及记忆功能.行为学试验结果表明:MC-LR 染毒剂量为2.00 μg/kg时可显著损伤大鼠的学习记忆能力及自发活动能力;核磁共振成像(MRI)试验结果显示, 染毒大鼠海马中的NAA/Cr比值显著降低(P<0.05).研究提示, 低剂量MC-LR能够导致神经行为及海马组织神经元的损伤.

References:

[1] Mezhoud K, Praseuth D, Puiseux-Dao S, et al. Global quantitative analysis of protein expression and phosphorylation status in the liver of the medaka fish(Oryzias latipes)exposed to microcystin-LR: Ⅰ. Balneation study [J]. Aquatic Toxicology, 2008, 86(2): 166-175.
[2] Dietrich D, Hoeger S. Guidance values for microcystins in water and cyanobacterial supplement products(blue-green algal supplements): a reasonable or misguided approach? [J]. Toxicology and Applied Pharmacology, 2005, 203(3): 273-289.
[3] Msagati T A, Siame B A, Shushu D D. Evaluation of methods for the isolation, detection and quantification of cyanobacterial hepatotoxins [J]. Aquatic Toxicology, 2006, 78(4): 382-397.
[4] Zegura B, Zajc I, Lah T T, et al. Patterns of microcystin-LR induced alteration of the expression of genes involved in response to DNA damage and apoptosis [J]. Toxicon, 2008, 51(4): 615-623.
[5] Fischer W J, Altheimer S, Cattori V, et al. Organic anion transporting polypeptides expressed in liver and brain mediate uptake of microcystin [J]. Toxicology and Applied Pharmacology, 2005, 203(3): 257-263.
[6] Jiang Y, Zheng W, Long L, et al. Brain magnetic resonance imaging and manganese concentrations in red blood cells of smelting workers: search for biomarkers of manganese exposure [J]. Neurotoxicology, 2007, 28(1): 126-135.
[7] Wang Q, Xie P, Chen J, et al. Distribution of microcystins in various organs(heart, liver, intestine, gonad, brain, kidney and lung)of Wistar rat via intravenous injection [J]. Toxicon, 2008, 52(6): 721-727.
[8] Baganz D, Staaks G, Pflugmacher S, et al. Comparative study of microcystin-LR-induced behavioral changes of two fish species, Danio rerio and Leucaspius delineatus [J]. Environ Toxicol, 2004, 19(6): 564-570.
[9] Bates T E, Strangward M, Keelan J, et al. Inhibition of N-acetylaspartate production: implications for 1H MRS studies in vivo [J]. Neuroreport, 1996, 7(8): 1397-1400.
[10] Xi G, Hui J, Zhang Z, et al. Learning and memory alterations are associated with hippocampal N-acetylaspartate in a rat model of depression as measured by 1H-MRS [J]. PloS One, 2011, 6(12): 28686-1-28686-9.
[11] Ju J, Ruan Q, Li X, et al. Neurotoxicological evaluation of microcystin-LR exposure at environmental relevant concentrations on nematode Caenorhabditis elegans [J]. Environmental Science and Pollution Research, 2013, 20(3): 1823-1830.
[12] Li X B, Zhang X, Ju J, et al. Alterations in neurobehaviors and inflammation in hippocampus of rats induced by oral administration of microcystin-LR [J]. Environ Sci Pollut Res, 2014, 21(21): 12419-12425.
[13] Loureiro M, Cholvin T, Lopez J, et al. The ventral midline thalamus(reuniens and rhomboid nuclei)contributes to the persistence of spatial memory in rats [J]. The Journal of Neuroscience, 2012, 32(29): 9947-9959.
[14] Frankland P W, Bontempi B. The organization of recent and remote memories [J]. Nature Reviews Neuroscience, 2005, 6(2): 119-130.
[15] Bernasconi N, Bernasconi A, Caramanos Z, et al. Mesial temporal damage in temporal lobe epilepsy: a volumetric MRI study of the hippocampus, amygdala and parahippocampal region [J]. Brain, 2003, 126(2): 462-469.
[16] Sierosawska A, Rymuszka A, Bownik A, et al. The influence of microcystin-LR on fish phagocytic cells [J]. Human & Experimental Toxicology, 2007, 26(7): 603-607.
[17] Meng X M, Zhu D M, Ruan D Y, et al. Effects of chronic lead exposure on 1H MRS of hippocampus and frontal lobes in children [J]. Neurology, 2005, 64(9): 1644-1647.
[18] Xu X, Xiang Y, Yang M. Subacute toxicity of exogenous manganese on rat hippocampal neurons [J]. Neural Regeneration Research, 2008, 3(5): 550-553.
[19] Ross A J, Sachdev P S. Magnetic resonance spectroscopy in cognitive research [J]. Brain Research Reviews, 2004, 44(2): 83-102.
[20] Sandi C. Stress, cognitive impairment and cell adhesion molecules [J]. Nature Reviews Neuroscience, 2004, 5(12): 917-930.
[21] Vorhees C V, Williams M T. Morris water maze: procedures for assessing spatial and related forms of learning and memory [J]. Nature Protocols, 2006, 1(2): 848-858.
[22] Dash P, Mach S, Moore A. Enhanced neurogenesis in the rodent hippocampus following traumatic brain injury [J]. Journal of Neuroscience Research, 2001, 63(4): 313-319.
[23] Maidana M, Carlis V, Galhardi F G, et al. Effects of microcystins over short-and long-term memory and oxidative stress generation in hippocampus of rats [J]. Chemico-Biological Interactions, 2006, 159(3): 223-234.
[24] Kondratova A A, Dubrovsky Y V, Antoch M P, et al. Circadian clock proteins control adaptation to novel environment and memory formation [J]. Aging, 2010, 2(5): 285-297.
[25] Van den Heuvel D M, Pasterkamp R J. Getting connected in the dopamine system [J]. Progress in Neurobiology, 2008, 85(1): 75-93.

Memo

Memo:

Biography: Li Yunhui(1968—), male, doctor, associate professor, yhli@seu.edu.cn.
Foundation item: The National Natural Science Foundation of China(No.81072258, 81273123).
Citation: Li Yunhui, Zhou Jue, Zhang Minhui, et al. Effects of microcystin-LR on hippocampal N-acetylaspartate and neurobehaviors in rats[J].Journal of Southeast University(English Edition), 2015, 31(4):577-581.[doi:10.3969/j.issn.1003-7985.2015.04.025]

Common functions