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[1] Wu Gensheng, Zhang Yin, Si Wei, Gu Yunfeng, et al. Current blockade mechanism for DNA translocationthrough solid-state nanopore with different membrane thickness [J]. Journal of Southeast University (English Edition), 2016, 32 (3): 301-306. [doi:10.3969/j.issn.1003-7985.2016.03.007]
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Current blockade mechanism for DNA translocationthrough solid-state nanopore with different membrane thickness()
不同薄膜厚度下的DNA分子通过固态纳米孔的 相对堵塞电流机理
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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:
32
Issue:
2016 3
Page:
301-306
Research Field:
Mathematics, Physics, Mechanics
Publishing date:
2016-09-20

Info

Title:
Current blockade mechanism for DNA translocationthrough solid-state nanopore with different membrane thickness
不同薄膜厚度下的DNA分子通过固态纳米孔的 相对堵塞电流机理
Author(s):
Wu Gensheng1;  Zhang Yin2;  Si Wei2;  Gu Yunfeng1;  Chen Yunfei2
1School of Mechanical and Electronic Engineering, Nanjing Forestry University, Nanjing 210037, China
2Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing 211189, China
伍根生1;  章寅2;  司伟2;  顾云风1;  陈云飞2
1南京林业大学机械电子工程学院, 南京 210037; 2东南大学江苏省微纳生物医疗器械设计与制造重点实验室, 南京 211189
Keywords:
nanopore;  DNA sequencing;  relative current blockade;  membrane thickness
纳米孔;  基因测序;  相对堵塞电流;  薄膜厚度
PACS:
O561.4
DOI:
10.3969/j.issn.1003-7985.2016.03.007
Abstract:
The current blockade mechanism for λ-DNA translocation under electrical field is investigated through solid-state nanopores with different pore thicknesses. The conductance of a nanopore system mainly consists of the contribution of the pore and access region, and the latter becomes dominant when the nanopore thickness gradually decreases to atomic layer thickness. Based on the existing model of nanopore resistance, a simplified model which describes the relative current blockade during the λ-DNA translocation through the nanopores is deduced to quantitatively present the relationship between nanopore thickness and relative current blockade. Results show that the relative current blockade is effectively increased by reducing the nanopore diameter but it decreases with the decreasing nanopore thickness. A two-stage schematic is proposed to increase the relative current blockade by setting a much smaller resistance region. Experimental results show a 21.9% increase in the relative current blockade with the proposed schematic.
通过改变固态纳米孔薄膜厚度, 揭示了λ-DNA在电场作用下通过纳米孔时堵塞离子电流的作用机理.纳米孔的电导主要由孔内电阻和孔口电阻构成, 当纳米孔长度逐渐减小到与原子层厚度相等时, 电导主要由孔口电阻主导.根据已有的纳米孔孔口电阻模型, 推导出一个简化的DNA分子堵塞纳米孔的相对离子电流模型, 定量地描述了纳米孔薄膜厚度与相对堵塞电流大小之间的关系.结果表明, 相对堵塞电流随着纳米孔直径的减小而增加, 但随着纳米孔厚度的减小而减小.为提高相对堵塞电流, 提出了一种二级结构, 该结构设置了一个更小的纳米孔孔口电阻区域.实验结果显示, 该结构使相对堵塞电流提高了21.9%.

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Memo

Memo:
Biography: Wu Gensheng(1986—), male, doctor, lecturer, genshengwu@126.com.
Foundation items: The Natural Science Foundation of Jiangsu Province(No.BK20160935), the Natural Science Foundation of Higher Education Institutions of Jiangsu Province(No.16KJB460015).
Citation: Wu Gensheng, Zhang Yin, Si Wei, et al.Current blockade mechanism for DNA translocation through solid-state nanopore with different membrane thickness[J].Journal of Southeast University(English Edition), 2016, 32(3):301-306.DOI:10.3969/j.issn.1003-7985.2016.03.007.
Last Update: 2016-09-20