[1] Yan Chunni, Huang Juan, Yang Sisi, Cao Chong, et al. Nitrification intensity and ammonia-oxidizing bacteria and archaeain different wetland plant rhizosphere soils [J]. Journal of Southeast University (English Edition), 2017, 33 (4): 466-472. [doi:10.3969/j.issn.1003-7985.2017.04.012]
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Nitrification intensity and ammonia-oxidizing bacteria and archaeain different wetland plant rhizosphere soils(
)
不同湿地植物根际硝化作用强度及氨氧化细菌和古菌
)Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]
- Volumn:
- 33
- Issue:
- 2017 4
- Page:
- 466-472
- Research Field:
- Environmental Science and Engineering
- Publishing date:
- 2017-12-30
Info
- Title:
- Nitrification intensity and ammonia-oxidizing bacteria and archaeain different wetland plant rhizosphere soils
- 不同湿地植物根际硝化作用强度及氨氧化细菌和古菌
- Author(s):
- Yan Chunni, Huang Juan, Yang Sisi, Cao Chong, Peng Cheng, Li Runqing
- School of Civil Engineering, Southeast University, Nanjing 210096, China
- Keywords:
- wetland plant rhizosphere; nitrification intensity; ammonia-oxidizing bacteria; ammonia-oxidizing archaea; fluorescence in situ hybridization
- PACS:
- X171.1
- DOI:
- 10.3969/j.issn.1003-7985.2017.04.012
- Abstract:
- In order to explore the nitrogen removal process in constructed wetlands(CWs), the moisture, ammonia nitrogen(NH4+-N), nitrate nitrogen(NO-3-N)and nitrification intensity in three wetland plant rhizosphere soils(Acorus calamus, Typha orientalis, Iris pseudacorus)were investi-gated at a relatively normal temperature range of 15 to 25 ℃. The relative abundance of ammonia-oxidizing bacteria(AOB)and ammonia-oxidizing archaea(AOA)were also achieved using fluorescence in situ hybridization(FISH). It is found that T.orientalis achieves the highest nitrification intensity of 2.03 mg/(h ·kg)while the second is I.pseudacorus(1.74 mg/(h ·kg)), and followed by A.calamus(1.65 mg/(h ·kg))throughout the experiment. FISH reveals that the abundance of bacteria(1010 g-1 wet soil)is higher than that of archaea(109 g-1 wet soil), and AOB are the dominant bacteria in the ammonia oxidation process. The abundance of AOB in the rhizosphere soils from high to low are T.orientalis(1.88×1010g-1), I.pseudacorus(1.23×1010 g-1), A.calamus(5.07×109 g-1)while the abundance of AOA from high to low are I.pseudacorus(4.00×109 g-1), A.calamus(3.52×109 g-1), T.orientalis(3.48×109 g-1). The study provides valuable evidence of plant selection for nitrogen removal in CWs.
- 为探究人工湿地脱氮进程, 选用菖蒲(Acorus calamus)、香蒲(Typha orientalis)、黄菖蒲(Iris pseudacorus)三种湿地植物, 于常温域15~25 ℃下测定其根际土壤含水率、氨氮、硝氮、硝化作用强度, 并采用荧光原位杂交(FISH)技术考察植物根际氨氧化细菌(AOB)和氨氧化古菌(AOA)的丰度变化规律.结果表明, 试验期间香蒲根际土壤的硝化作用强度最高, 平均为2.03 mg/(h·kg), 其次为黄菖蒲1.74 mg/(h·kg)和菖蒲1.65 mg/(h·kg).FISH技术表明湿地植物根际土壤中的细菌数量(数量级为1010)高于古菌(数量级为109), AOB为氨氧化过程的优势菌群.3种湿地植物根际AOB的数量从高到低(以湿土计)依次为:香蒲(1.88×1010g-1)、黄菖蒲(1.23×1010 g-1)、菖蒲(5.07×109 g-1);AOA的数量从高到低(以湿土计)依次为:黄菖蒲(4.00×109 g-1)、菖蒲(3.52×109 g-1)、香蒲(3.48×109 g-1).该试验结论为人工湿地脱氮的植物选择提供了有价值的参考.
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Memo
- Memo:
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Biographies: Yan Chunni(1989—), female, master; Huang Juan(corresponding author), female, doctor, professor, 101010942@seu.edu.cn.
Foundation items: The National Natural Science Foundation of China(No.51479034, 50909019), the Fundamental Research Funds for the Central Universities.
Citation: Yan Chunni, Huang Juan, Yang Sisi, et al. Nitrification intensity and ammonia-oxidizing bacteria and archaea in different wetland plant rhizosphere soils[J].Journal of Southeast University(English Edition), 2017, 33(4):466-472.DOI:10.3969/j.issn.1003-7985.2017.04.012.