|Table of Contents|

[1] Yan Chunni, Huang Juan, Yang Sisi, Cao Chong, et al. Nitrification intensity and ammonia-oxidizing bacteria and archaeain different wetland plant rhizosphere soils [J]. Journal of Southeast University (English Edition), 2017, 33 (4): 466-472. [doi:10.3969/j.issn.1003-7985.2017.04.012]
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Nitrification intensity and ammonia-oxidizing bacteria and archaeain different wetland plant rhizosphere soils()
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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:
33
Issue:
2017 4
Page:
466-472
Research Field:
Environmental Science and Engineering
Publishing date:
2017-12-30

Info

Title:
Nitrification intensity and ammonia-oxidizing bacteria and archaeain different wetland plant rhizosphere soils
Author(s):
Yan Chunni;  Huang Juan;  Yang Sisi;  Cao Chong;  Peng Cheng;  Li Runqing
School of Civil Engineering, Southeast University, Nanjing 210096, China
Keywords:
wetland plant rhizosphere;  nitrification intensity;  ammonia-oxidizing bacteria;  ammonia-oxidizing archaea;  fluorescence in situ hybridization
PACS:
X171.1
DOI:
10.3969/j.issn.1003-7985.2017.04.012
Abstract:
In order to explore the nitrogen removal process in constructed wetlands(CWs), the moisture, ammonia nitrogen(NH4+-N), nitrate nitrogen(NO-3-N)and nitrification intensity in three wetland plant rhizosphere soils(Acorus calamus, Typha orientalis, Iris pseudacorus)were investi-gated at a relatively normal temperature range of 15 to 25 ℃. The relative abundance of ammonia-oxidizing bacteria(AOB)and ammonia-oxidizing archaea(AOA)were also achieved using fluorescence in situ hybridization(FISH). It is found that T.orientalis achieves the highest nitrification intensity of 2.03 mg/(h ·kg)while the second is I.pseudacorus(1.74 mg/(h ·kg)), and followed by A.calamus(1.65 mg/(h ·kg))throughout the experiment. FISH reveals that the abundance of bacteria(1010 g-1 wet soil)is higher than that of archaea(109 g-1 wet soil), and AOB are the dominant bacteria in the ammonia oxidation process. The abundance of AOB in the rhizosphere soils from high to low are T.orientalis(1.88×1010g-1), I.pseudacorus(1.23×1010 g-1), A.calamus(5.07×109 g-1)while the abundance of AOA from high to low are I.pseudacorus(4.00×109 g-1), A.calamus(3.52×109 g-1), T.orientalis(3.48×109 g-1). The study provides valuable evidence of plant selection for nitrogen removal in CWs.

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Memo

Memo:
Biographies: Yan Chunni(1989—), female, master; Huang Juan(corresponding author), female, doctor, professor, 101010942@seu.edu.cn.
Foundation items: The National Natural Science Foundation of China(No.51479034, 50909019), the Fundamental Research Funds for the Central Universities.
Citation: Yan Chunni, Huang Juan, Yang Sisi, et al. Nitrification intensity and ammonia-oxidizing bacteria and archaea in different wetland plant rhizosphere soils[J].Journal of Southeast University(English Edition), 2017, 33(4):466-472.DOI:10.3969/j.issn.1003-7985.2017.04.012.
Last Update: 2017-12-20