[1] Zhang Xinjie, Zhu Hangjie, Liu Yao, et al. Stepped spiral microchannels for rapid blood plasma separation [J]. Journal of Southeast University (English Edition), 2023, 39 (2): 176-186. [doi:10.3969/j.issn.1003-7985.2023.02.009]

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Stepped spiral microchannels for rapid blood plasma separation()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

39

Issue:

2023 2

Page:

176-186

Research Field:

Mechanical Engineering

Publishing date:

2023-06-20

Info

Title:

Stepped spiral microchannels for rapid blood plasma separation

Author(s):

Zhang Xinjie¹;  2;  Zhu Hangjie¹;  Liu Yao¹;  Gu Qiao³;  Zhang Yuhang¹Oseyemi Ayobami Elisha⁴;  Lü Fangrui¹;  Ni Zhonghua²

¹College of Mechanical and Electrical Engineering, Hohai University, Changzhou 213022, China
²School of Mechanical Engineering, Southeast University, Nanjing 211189, China
³Department of Gynecology and Obstetrics, The Third Affiliated Hospital of Soochow University, Changzhou 213003, China
⁴Department of Mechanical Engineering, York University, Toronto, ON M3J1P3, Canada

Keywords:

microfluidics;  inertial focusing;  stepped spiral channel;  blood plasma separation;  secondary flow regulation

PACS:

TH77

DOI:

10.3969/j.issn.1003-7985.2023.02.009

Abstract:

Conventional ways of blood processing, such as centrifugation and filtration, are fairly limited by processing time, separation purity, clogging, and other factors. To solve this problem, a high-throughput, inertial microfluidic device composed of a stepped spiral channel is proposed for the separation of plasma from high-concentration blood. First, the particle-focusing characteristics of the stepped spiral channel are studied through the coupling of laminar flow and particle tracking modules in the COMSOL Multiphysics^�AE; software. Next, polystyrene beads are used to investigate the inertial focusing performances of the stepped spiral channel at different flow rates. Based on the experimental results of particle focusing, an optimized stepped spiral channel is applied in blood plasma separation from samples of different cell concentrations. It can be observed that the reject ratios of the blood cells are(99.72±0.13)% and(99.44±0.17)% with hematocrit(HCT)values of 0.9% and 2.25%, respectively, at an optimal flow rate of 1.5 mL/min. The ratios of blood cells rejected by the stepped spiral channel are(97.02±0.56)% and(92.92±1.53)% at ultrahigh HCT values of 4.5% and 9%, respectively. The experimental findings demonstrate that the stepped spiral channel can efficiently separate plasma from ultrahigh-concentration blood samples.

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Memo

Memo:

Biography: Zhang Xinjie(1984—), male, doctor, associate professor, xj.zhang@hhu.edu.cn.
Foundation items: The National Natural Science Foundation of China(No.51905150), Fundamental Research Funds for the Central Universities(No.B220202024), Changzhou Science and Technology Bureau Program(No.CE20225046), Changzhou Health Commission Youth Science and Technology Projects(No.QN202115), Jiangsu Planned Projects for Postdoctoral Research Funds(No.2019K033), Postgraduate Research and Practice Innovation Program of Jiangsu Province(No.KYCX23_0670).
Citation: Zhang Xinjie, Zhu Hangjie, Liu Yao, et al. Stepped spiral microchannels for rapid blood plasma separation[J].Journal of Southeast University(English Edition), 2023, 39(2):176-186.DOI:10.3969/j.issn.1003-7985.2023.02.009.

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