[1] Nie Libo, Chen Hong, Tan Meijun, et al. Improvement of hybridization signalsof gold label silver stain gene detection [J]. Journal of Southeast University (English Edition), 2004, 20 (4): 463-466. [doi:10.3969/j.issn.1003-7985.2004.04.014]

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Improvement of hybridization signalsof gold label silver stain gene detection()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

20

Issue:

2004 4

Page:

463-466

Research Field:

Chemistry and Chemical Engineering

Publishing date:

2004-12-30

Info

Title:

Improvement of hybridization signalsof gold label silver stain gene detection

Author(s):

Nie Libo¹;  Chen Hong²;  3;  Tan Meijun³;  He Nongyue¹

¹Key Laboratory of Molecular and Biomolecular Electronics of Ministry of Education, Southeast University, Nanjing 210096, China
²Department of Life Science and Technology, Central South Forestry University, Changsha 410004, China
³School of Packaging and Printing, Zhuzhou Institute of Technology, Zhuzhou 412008, China

Keywords:

gold label silver stain;  gold nanoparticle;  hybridization signal

PACS:

O633

DOI:

10.3969/j.issn.1003-7985.2004.04.014

Abstract:

The factors that influence the colorimetric gene detection of gold label silver stain and improve the detection signals are studied. The influence of amino DNA probes and thiol DNA modified gold nanoparticles is investigated based on a sandwich hybridization system. An increase in amino probe concentration brings about an increase in hybridization signal which reaches a threshold corresponding to the saturated concentration of amino probes bounded onto a glass slide surface. Since the steric hindrance effect of nanoparticles is dominant over the influence of a surface area, the bigger gold nanoparticles lead to weaker hybridization signals. The hybridization efficiency enhances significantly with the increase of the thiol DNA modified nanoparticle concentrations. Experimental results show that 125 μmol/L of the amino DNA probe concentration, 15 nm of the gold nanoparticle diameter, and 4. 07 nmol/L of the thiol DNA modified gold nanoparticle concentration are optimal for the detection system. The hybridization signals can be improved remarkably by choosing optimal hybridization conditions.

References:

[1] Storhoff J J, Elghanian R, Mucic R C, et al. One-pot colorimetric differentiation of polynucleotides with single base imperfections using gold nanoparticle probes [J]. J Am Chem Soc, 1998, 120(9): 1959-1964.
[2] Mucic R C, Storhoff J J, Mirkin C A, et al. DNA-directed synthesis of binary nanoparticle network materials [J]. J Am Chem Soc, 1998, 120(48): 12674-12675.
[3] Elghanian R, Storhoff J J, Mucic R C, et al. Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles [J]. Science, 1997, 277: 1078-1081.
[4] Taton T A, Mucic R C, Mirkin C A, et al. The DNA-mediated formation of supramolecular mono-and multilayered nanoparticle structures [J]. J Am Chem Soc, 2000, 122(26): 6305-6306.
[5] Taton T A, Lu G, Mirkin C A. Two-color labeling of oligonucleotide arrays via size-selective scattering of nanoparticle probes [J]. J Am Chem Soc, 2001, 123(21): 5164-5165.
[6] Taton T A, Mirkin C A, Letsinger R L, et al. Scanometric DNA array detection with nanoparticle probes [J]. Science, 2000, 289: 1757-1760.
[7] Nie Libo, Tang Jianxin, Guo Huishi, et al. Colorimetric detection of polynucleotides on polypropylene slices [J]. Analytical Sciences, 2004, 20(3): 461-463.

Memo

Memo:

Biographies: Nie Libo(1973—), female, graduate; He Nongyue(corresponding author), male, doctor, professor, nyhe1958@163.com.

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