[1] Wang Ziyu, Zhang Dongsheng, Gu Ning, Lu Xinli, et al. Preparation and evaluation of As2O3 nanoparticlesfor treatment of human liver cancer cells in vitro [J]. Journal of Southeast University (English Edition), 2005, 21 (1): 58-62. [doi:10.3969/j.issn.1003-7985.2005.01.013]

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Preparation and evaluation of As₂O₃ nanoparticlesfor treatment of human liver cancer cells in vitro()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

21

Issue:

2005 1

Page:

58-62

Research Field:

Biological Science and Medical Engineering

Publishing date:

2005-03-30

Info

Title:

Preparation and evaluation of As₂O₃ nanoparticlesfor treatment of human liver cancer cells in vitro

Author(s):

Wang Ziyu¹;  Zhang Dongsheng¹;  Gu Ning²;  Lu Xinli¹;  Yan Shiyan¹

¹School of Basic Medical Science, Southeast University, Nanjing 210009, China
²Department of Biomedical Science and Engineering, Southeast University, Nanjing 210096, China

Keywords:

nanotechnology;  arsenic trioxide;  liver cancer

PACS:

R73-36

DOI:

10.3969/j.issn.1003-7985.2005.01.013

Abstract:

This paper studies the preparation method of As₂O₃ nanoparticles and their antitumor effect on human liver cancer cells.As₂O₃ nanoparticles were prepared by sol-gel method.As₂O₃ nanoparticles were characterized by transmission electron microscope(TEM), energy dispersive spectrometer(EDS)and computer color magic image analysis system(CMIAS).A methyl thiazolyl tetrazolium(MTT)assay and a flow cytometry(FCM)assay were performed to examine the antitumor effect of As₂O₃ nanoparticles at various concentrations(1, 2, 5, 10 μmol/L).We also compared the antitumor effect of As₂O₃ nanoparticles with that of As₂O₃ solution.The average diameters of two kinds of As₂O₃ nanoparticles prepared were about 80 nm and 40 nm.It was identified that the prepared nanoparticles were As₂O₃ and there were no other components by EDS.After 48 h of treatment with As₂O₃ nanoparticles, the survival ratio of cells was significantly lower than that of the As₂O₃ solution with the same concentration(P<0.05).Experimental results demonstrate that by sol-gel method As₂O₃ can be prepared into nanoparticles.As₂O₃ nanoparticles can produce a better cytotoxic effect on tumor cells than the As₂O₃ solution.

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Memo

Memo:

Biographies: Wang Ziyu(1979—), female, graduate;Zhang Dongsheng(corresponding author), male, professor, b7712900@jlonline.com.

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