|Table of Contents|

[1] Pan Haixian, Lü Xiaoying, Wang Zhigong, Fang Tao, et al. Microelectrode array for bioelectrical signal stimulation and recording [J]. Journal of Southeast University (English Edition), 2011, 27 (4): 361-366. [doi:10.3969/j.issn.1003-7985.2011.04.003]

Microelectrode array for bioelectrical signal stimulation and recording()

Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

2011 4
Research Field:
Circuit and System
Publishing date:


Microelectrode array for bioelectrical signal stimulation and recording
Pan Haixian1 Lü Xiaoying1 Wang Zhigong2 Fang Tao1 Qiu Lei2 Huang Zonghao2
1 State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
2 Institute of RF- & OE-ICs, Southeast University, Nanjing 210096, China
microelectrode array(MEA) stimulation and recording extracellular recording toad sciatic nerve
A microelectrode array(MEA)is presented, which is composed of 60 independent electrodes with 59 working ones and one reference one, and they are divided into 30 pairs. Except for the reference electrode, each pair consists of one stimulating electrode and one recording electrode. Supported by the peripheral circuits, four electrode states to study the bioelectrical signal of biological tissue or slice cultured in-vitro on the surface of the electrodes can be realized through each pair of electrodes. The four electrode states are stimulation, recording, stimulation and recording simultaneously, and isolation. The state of each pair of working electrodes can be arbitrarily controlled according to actual needs. The MEAs are fabricated in printed circuit board(PCB)technology. The total area of the PCB-based MEA is 49 mm×49 mm. The impedance measurement of MEA is carried out in 0.9% sodium chloride solution at room temperature by means of 2-point measurements with an Agilent LCR meter, and the test signal for the impedance measurement is sinusoidal(AC voltage 50 mV, sweeping frequency 20 Hz to 10 kHz). The electrode impedance is between 200 and 3 kΩ while the frequency is between 500 and 1 000 Hz. The electrode impedance magnitude is inversely proportional to the frequency. Experiments of toad sciatic nerve in-vitro stimulation and recording and signal regeneration between isolated toad sciatic nerves are carried out on the PCB-based MEA. The results show that the MEA can be used for bioelectrical signal stimulation, recording, stimulation and recording simultaneously, and isolation of biological tissues or slices in-vitro.


[1] Hoag H. Neuroengineering: remote control[J]. Nature, 2003, 423(6942): 796-798.
[2] Eversmann B, Jenkner M, Hofmann F, et al. A 128×128 CMOS biosensor array for extracellular recording of neural activity[J]. IEEE J Solid-State Circuits, 2003, 38(12):2306-2317.
[3] Heer F, Franks W, Blau A, et al. CMOS microelectrode array for the monitoring of electrogenic cells[J]. Biosens Bioelectron, 2004, 20(2): 358-386.
[4] Heer F, Hafizovic S, Franks W, et al. CMOS microelectrode array for bidirectional interaction with neuronal networks[J]. IEEE J Solid-State Circuits, 2006, 41(7):1620-1629.
[5] Xiang G X, Pan L B, Huang L H, et al. Microelectrode array-based system for neuropharmacological applications with cortical neurons cultured in vitro[J]. Biosens Bioelectron, 2007, 22(11): 2478-2484.
[6] Neher E, Sakmann B. Single-channel currents recorded from membrane of denervated frog muscle fibres[J]. Nature, 1976, 260(5554): 799-802.
[7] Cole K. Dynamic electrical characteristics of the squid axon membrane[J]. Arch Sci Physiol, 1949, 3: 253-258.
[8] Thomas C A, Springer P A, Loeb G E, et al. A miniature microelectrode array to monitor the bioelectrical activity of cultured cells[J]. Exp Cell Res, 1972, 74(1): 61-66.
[9] Liu Q J, Ye W W, Xiao L D, et al. Extracellular potentials recording in intact olfactory epithelium by microelectrode array for a bioelectronic nose[J]. Biosens Bioelectron, 2010, 25(10):2212-2217.
[10] Berdondini L, Massobrio P, Chiappalone M, et al. Extracellular recordings from locally dense microelectrode arrays coupled to dissociated cortical cultures[J]. J Neurosci Meth, 2009, 177(2): 386-396.
[11] Hill A J, Jones N A, Williams C M, et al. Development of multi-electrode array screening for anticonvulsants in acute rat brain slices[J].J Neurosci Meth, 2010, 185(2): 246-256.
[12] Frey U, Heer F, Pedron R, et al. An 11k-electrode 126-channel high-density microelectrode array to interact with electrogenic cells[C]//IEEE International Solid-State Circuits Conference. San Francisco, CA, USA, 2007: 158-160.
[13] Xu G X, Ye X S, Qin L F, et al. Cell-based biosensors based on light-addressable potentiometric sensors for single cell monitoring[J]. Biosens Bioelectron, 2005, 20(9):1757-1763.
[14] Chen C H, Yao D J, Tseng S H, et al. Micro-multi-probe electrode array to measure neural signals[J]. Biosens Bioelectron, 2009, 24(7): 1911-1917.
[15] Pan H X, Lü X Y, Wang Z G, et al. Silicon-based microelectrode arrays for stimulation and signal recording of in vitro cultured neurons[J].Sci China Ser F: Info Sci, 2011, 54(10): 2199-2208.
[16] Lin J H, Wu X M, Huang P S, et al. Development of silicon-based microelectrode array[J]. Sci China Ser E: Tech Sci, 2009, 52(8): 2391-2395.
[17] Pan H X, Lü X Y, Wang Z G, et al. Microelectrode array for detecting electrical activities of neuron assemble[J]. Journal of Southeast University: Natural Science Edition, 2009, 39(3): 468-472.(in Chinese)


Biographies: Pan Haixian(1983—), female, graduate; Lü Xiaoying(corresponding author), female, doctor, professor, luxy@seu.edu.cn.
Foundation items: The National Natural Science Foundation of China(No.61076118, 90307013, 90707005), the Natural Science Foundation of Jiangsu Province(No.BK2008032), Special Foundation and Open Foundation of the State Key Laboratory of Bioelectronics of Southeast University.
Citation: Pan Haixian, Lü Xiaoying, Wang Zhigong, et al.Microelectrode array for bioelectrical signal stimulation and recording[J].Journal of Southeast University(English Edition), 2011, 27(4):361-366.[doi:10.3969/j.issn.1003-7985.2011.04.003]
Last Update: 2011-12-20