[1] Si WeiYang HaojieJi AnpingLi Kun, Sha JingjieLiu Lei, Chen Yunfei,. Electrophoresis of poly(dT)20 through α-hemolysin nanoporein high concentration potassium chloride solution [J]. Journal of Southeast University (English Edition), 2016, 32 (4): 496-501. [doi:10.3969/j.issn.1003-7985.2016.04.017]

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Electrophoresis of poly(dT)₂₀ through α-hemolysin nanoporein high concentration potassium chloride solution()

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

32

Issue:

2016 4

Page:

496-501

Research Field:

Mechanical Engineering

Publishing date:

2016-12-20

Info

Title:

Electrophoresis of poly(dT)₂₀ through α-hemolysin nanoporein high concentration potassium chloride solution

Author(s):

Si WeiYang HaojieJi AnpingLi Kun;  Sha JingjieLiu Lei;  Chen Yunfei

School of Mechanical Engineering, Southeast University, Nanjing 211189, China
Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Nanjing 211189, China

Keywords:

nanopore;  transport speed;  ionic current;  electrophoresis

PACS:

TH789;Q786

DOI:

10.3969/j.issn.1003-7985.2016.04.017

Abstract:

Experiments of poly(dT)₂₀ electrophoresis through α-hemolysin nanopores were performed to unveil the electrophoretic transport mechanism of DNA through nanopores in high concentration potassium chloride solution. It is found that there are two obvious current blockades induced by poly(dT)₂₀ translocation and collision events. Both blockade currents increase linearly with the applied bias voltage. However, the normalized blockade currents are almost kept the same although variable bias voltages are applied. The collision time of poly(dT)₂₀ in the luminal site of the pore remains constant for different voltages. The translocation speed of poly(dT)₂₀ through the nanopore decreases with the increase of bias voltage. It is because as the potential increases, the drag force on the homopolymer helps it to crumple into a cluster much easier due to the poor stacking of thymine residues compared with homopolymers consisting of other nucleotides. Molecular dynamics simulations further confirm the experimental results. Increasing the applied bias voltage can slow down the translocation velocity of the flexible poly(dT)₂₀, which favors increasing the precision of single molecule detection by using nanopores.

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Memo

Memo:

Biographies: Si Wei(1989—), male, graduate; Chen Yunfei(corresponding author), male, doctor, professor, yunfeichen@seu.edu.cn.
Foundation items: The National Natural Science Foundation of China(No.51435003, 51375092), Research Program of Chongqing Municipal Education Commission(No.KJ1401030), the Research & Innovation Program for Graduate Student in Universities of Jiangsu Province(No.KYLX_0100), the Scientific Research Foundation of Graduate School of Southeast University(No.YBJJ1540).
Citation: Si Wei, Yang Haojie, Ji Anping, et al.Electrophoresis of poly(dT)₂₀ through α-hemolysin nanopore in high concentration potassium chloride solution[J].Journal of Southeast University(English Edition), 2016, 32(4):496-501.DOI:10.3969/j.issn.1003-7985.2016.04.017.

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