[1] Han Yuexia, , He Weinan, et al. Intracellular temperature measurementby a dual-thermocouple difference method [J]. Journal of Southeast University (English Edition), 2021, 37 (1): 1-7. [doi:10.3969/j.issn.1003-7985.2021.01.001]

Copy

Intracellular temperature measurementby a dual-thermocouple difference method()

Share：

Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

37

Issue:

2021 1

Page:

1-7

Research Field:

Biological Science and Medical Engineering

Publishing date:

2021-03-20

Info

Title:

Intracellular temperature measurementby a dual-thermocouple difference method

Author(s):

Han Yuexia¹;  2;  3;  He Weinan¹;  2;  3;  Yang Sheng¹;  2;  3;  Mao Wei¹;  2;  3;  Gu Ning¹;  2;  3;  4

¹School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
²State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
³Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing 210096, China
⁴School of Biomedical Engineering and Informatics, Nanjing Medical University, Nanjing 211166, China

Keywords:

dual-thermocouple;  difference thermometry;  intracellular temperature;  nanotechnology

PACS:

Q28

DOI:

10.3969/j.issn.1003-7985.2021.01.001

Abstract:

A dual thermocouple difference technique is developed to determine the accuracy and anti-interference ability in the process of intracellular temperature measurement. First, two micro-nano thermocouples(TC)and a high-precision signal acquisition module are used to measure the temperature difference between the cell and the culture medium(separated about 10 μm from the cell). The cold junctions of two TCs are connected to eliminate the setting of the reference temperature and enhance the anti-interference ability. Then, a low-noise voltage amplifier and digital acquisition card are used to sample signals. In order to verify the feasibility of the dual thermocouple difference method, the temperature changes of U251 cells are detected. The calibration results of two TCs show that the Seebeck coefficient is about 5 μV/℃, and the signal acquisition accuracy is 0.5 μV in a low voltage range(0-15 μV). With the dual thermocouple difference method, errors due to the cold junctions can be removed and the interference caused by environmental temperature fluctuation can be reduced. The phenomenon of cellular temperature increase proves that the dual thermocouple difference method can detect the tiny temperature change of a single cell. The method potentially is a highly powerful technique for studying local thermogenesis of cells and helps to explore the relationship between cellular thermogenesis and cellular processes.

References:

[1] Donner J S, Thompson S A, Kreuzer M P, et al. Mapping intracellular temperature using green fluorescent protein[J].Nano Letters, 2012, 12(4): 2107-2111. DOI:10.1021/nl300389y.
[2] Maksimov E G, Yaroshevich I A, Tsoraev G V, et al. A genetically encoded fluorescent temperature sensor derived from the photoactive orange carotenoid protein[J].Scientific Reports, 2019, 9: 8937. DOI:10.1038/s41598-019-45421-7.
[3] Kucsko G, Maurer P C, Yao N Y, et al. Nanometre-scale thermometry in a living cell[J].Nature, 2013, 500(7460): 54-58. DOI:10.1038/nature12373.
[4] Albers A E, Chan E M, McBride P M, et al. Dual-emitting quantum dot/quantum rod-based nanothermometers with enhanced response and sensitivity in live cells[J].Journal of the American Chemical Society, 2012, 134(23): 9565-9568. DOI:10.1021/ja302290e.
[5] Okabe K, Sakaguchi R, Shi B N, et al. Intracellular thermometry with fluorescent sensors for thermal biology[J].Pflügers Archiv—European Journal of Physiology, 2018, 470(5): 717-731. DOI:10.1007/s00424-018-2113-4.
[6] Suzuki M, Plakhotnik T. The challenge of intracellular temperature[J].Biophysical Reviews, 2020, 12(2): 593-600. DOI:10.1007/s12551-020-00683-8.
[7] Brites C D S, Lima P P, Silva N J O, et al. Thermometry at the nanoscale[J].Nanoscale, 2012, 4(16): 4799-4829. DOI:10.1039/c2nr30663h.
[8] Zhou J J, del Rosal B, Jaque D, et al. Advances and challenges for fluorescence nanothermometry[J].Nature Methods, 2020, 17(10): 967-980. DOI:10.1038/s41592-020-0957-y.
[9] Gota C, Okabe K, Funatsu T, et al. Hydrophilic fluorescent nanogel thermometer for intracellular thermometry[J].Journal of the American Chemical Society, 2009, 131(8): 2766-2767. DOI:10.1021/ja807714j.
[10] Okabe K, Inada N, Gota C, et al. Intracellular temperature mapping with a fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy[J].Nature Communications, 2012, 3: 705. DOI:10.1038/ncomms1714.
[11] Suzuki M, Tseeb V, Oyama K, et al. Microscopic detection of thermogenesis in a single HeLa cell[J].Biophysical Journal, 2007, 92(6): L46-L48. DOI:10.1529/biophysj.106.098673.
[12] Wang C, Xu R, Tian W, et al. Determining intracellular temperature at single-cell level by a novel thermocouple method[J].Cell Research, 2011, 21(10): 1517-1519. DOI:10.1038/cr.2011.117.
[13] Tian W J, Wang C L, Wang J Q, et al. A high precision apparatus for intracellular thermal response at single-cell level[J].Nanotechnology, 2015, 26(35): 355501. DOI:10.1088/0957-4484/26/35/355501.
[14] Bai T T, Gu N. Micro/nanoscale thermometry for cellular thermal sensing[J].Small, 2016, 12(34): 4590-4610. DOI:10.1002/smll.201600665.
[15] Yang S, He W N, Li C, et al. A new approach of electrochemical etching fabrication based on drop-off-delay control[J].The Review of Scientific Instruments, 2019, 90(7): 074902. DOI:10.1063/1.5094470.
[16] Kallerhoff M, Karnebogen M, Singer D, et al. Microcalorimetric measurements carried out on isolated tumorous and nontumorous tissue samples from organs in the urogenital tract in comparison to histological and impulse-cytophotometric investigations[J].Urological Research, 1996, 24(2): 83-91. DOI:10.1007/BF00431084.
[17] Deberardinis R J, Lum J J, Hatzivassiliou G, et al. The biology of cancer: Metabolic reprogramming fuels cell growth and proliferation[J].Cell Metabolism, 2008, 7(1): 11-20. DOI:10.1016/j.cmet.2007.10.002.
[18] Inomata N, Van Toan N, Ono T. Liquid thermocouple using thermoelectric ionic liquids[J].IEEE Sensors Letters, 2019, 3(5): 1-4. DOI:10.1109/LSENS.2019.2912418.
[19] Yamamura M, Hayatsu H, Miyamae T. Heat production as a cell cycle monitoring parameter[J].Biochemical and Biophysical Research Communications, 1986, 140(1): 414-418. DOI:10.1016/0006-291X(86)91106-X.
[20] Lowell B B, Spiegelman B M. Towards a molecular understanding of adaptive thermogenesis[J].Nature, 2000, 404(6778): 652-660. DOI:10.1038/35007527.

Memo

Memo:

Biographies: Han Yuexia(1981—), male, doctor; Gu Ning(corresponding author), male, doctor, professor, 101001711@seu.edu.cn.
Foundation items: The National Key Research and Development Program of China(No.2017YFA0104302), the National Natural Science Foundation of China(No.61420106012, 61821002).
Citation: Han Yuexia, He Weinan, Yang Sheng, et al. Intracellular temperature measurement by a dual-thermocouple difference method[J].Journal of Southeast University(English Edition), 2021, 37(1):1-7.DOI:10.3969/j.issn.1003-7985.2021.01.001.

Common functions