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[1] Zhang Haiqian, *, Shinohara Hiroaki, Gu Ning, et al. Cell Adhesion Inhibition by RGD Peptides Linkedwith a Photoisomerizable Nonnatural Amino Acid [J]. Journal of Southeast University (English Edition), 2001, 17 (2): 22-26. [doi:10.3969/j.issn.1003-7985.2001.02.006]
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Cell Adhesion Inhibition by RGD Peptides Linkedwith a Photoisomerizable Nonnatural Amino Acid()
连接光异构化非天然氨基酸的RGD肽抑制细胞的附着
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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:
17
Issue:
2001 2
Page:
22-26
Research Field:
Biological Science and Medical Engineering
Publishing date:
2001-12-30

Info

Title:
Cell Adhesion Inhibition by RGD Peptides Linkedwith a Photoisomerizable Nonnatural Amino Acid
连接光异构化非天然氨基酸的RGD肽抑制细胞的附着
Author(s):
Zhang Haiqian1, 2*, Shinohara Hiroaki2, Gu Ning1, Sasaki Hiroshi2, Sisido Masahiko2
1Laboratory of Wu Chien-shiung, Southeast University, Nanjing 210096, China
2Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Okayama 700-8530, Japan
张海黔1, 2, NB328原宽明2, 顾宁1, 佐々木裕司2, 户昌彦2
1吴健雄实验室, 东南大学, 南京210096; 2生物科学与技术系, 冈山大学, 冈山700-8530, 日本
Keywords:
nonnatural amino acid;  cell adhesion;  photoisomerization;  integrin;  peptide
非天然氨基酸;  细胞附着;  光致异构化;  肽
PACS:
Q517;Q518.4
DOI:
10.3969/j.issn.1003-7985.2001.02.006
Abstract:
RGD peptides linked with a nonnatural amino acid, phenylazophenyl alaninine(azoAla), were synthesized and applied to cell adhesion inhibitors. The RGD peptides linked with azoAla at C-terminal showed potent binding to the integrin on the surface of HeLa cells. Photoisomerization effect of the azobenzene side chain of synthesized peptides on the cell adhesion inhibition was further investigated. It was demonstrated that the cis-form of azoAla-linked RGD peptides revealed a little weak cell adhesion inhibition effect as compared with trans-form of azoAla-linked RGD peptide.
利用固相肽合成方法, 将带有光敏基团偶氮苯的非天然氨基酸——苯基偶氮苯基苯丙氨酸与RGD肽连接, 得到具有光敏功能的RGD肽.非天然氨基酸连接在C-端的RGD肽与HeLa细胞表面的整合素有很强的结合能力.RGD侧链上的光敏基团的光致异构化对RGD肽与HeLa细胞表面的整合素的结合有一定的影响.

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Memo

Memo:
* Born in 1963, male, associate professor, doctor.
Last Update: 2001-06-20