[1] Zhu Chongzhi, Zhang Xiaodong, Sun Liwei, et al. Development and characterization of a functional microbialconsortium for crude oil degradation [J]. Journal of Southeast University (English Edition), 2021, 37 (4): 388-393. [doi:10.3969/j.issn.1003-7985.2021.04.007]

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Development and characterization of a functional microbialconsortium for crude oil degradation()

原油降解的功能微生物菌群的开发与性能分析

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Journal of Southeast University (English Edition)[ISSN:1003-7985/CN:32-1325/N]

Volumn:

37

Issue:

2021 4

Page:

388-393

Research Field:

Environmental Science and Engineering

Publishing date:

2021-12-20

Info

Title:

Development and characterization of a functional microbialconsortium for crude oil degradation

原油降解的功能微生物菌群的开发与性能分析

Author(s):

Zhu Chongzhi^{1, 2}, Zhang Xiaodong^{1, 2}, Sun Liwei¹, Zhan Manjun³, Long Tao², Yu Ran¹

¹ School of Energy and Environment, Southeast University, Nanjing 210096, China
² Nanjing Institute of Environmental Sciences of Ministry of Ecology and Environment, Nanjing 210019, China
³ Nanjing Research Institute of Environmental Protection, Nanjing 210013, China

祝冲之^{1, 2}, 张晓东^{1, 2}, 孙丽伟¹, 展漫军³, 龙涛², 余冉¹

¹ 东南大学能源与环境学院, 南京 210096; ² 生态环境部南京环境科学研究所, 南京 210019; ³ 南京市环境保护科学研究院, 南京 210013

Keywords:

crude oil;  biodegradation;  microbial consortium;  refractory residues

原油;  生物降解;  微生物菌群;  原油残渣

PACS:

X592

DOI:

10.3969/j.issn.1003-7985.2021.04.007

Abstract:

Crude oil-degrading microbial consortia were enriched from three oil-contaminated sites to achieve the efficient biodegradation of crude oil, especially its refractory residues. The gravimetric method was used to analyze the degradation efficiency of the enriched consortia and changes in the fractions of the crude oil. The effects of changes in environmental factors were also studied to determine the optimal oil-reducing conditions and assess the dominant bacteria of the mixed flora. Results show that all three consortia exhibit reliable crude oil-biodegradation abilities and that their mixture results in biodegradation rate are as high as(48.0±3.5)% over 30 d of incubation. The consortium mixture can degrade 11.1% of the refractory resins, 79.7% of the saturated hydrocarbons, and 45.7% of the aromatics in crude oil. Neutral pH, an incubation temperature of 30 ℃, and low mineral salt concentrations(0.8% to 4.0%)are optimal for crude oil biodegradation. The dominant genera in the consortium mixture include Pseudomonas, Stenotrophomonas, Brucella, Serratia, Brevundimonas, and Achromobacter. The richness and diversity of the microbial community in the consortium remain stable during crude oil degradation. Therefore, microbial enrichment from multiple sources may be performed to construct a mixed consortium for crude oil pollution bioremediation.

为了实现原油的生物降解, 从3个石油污染场地的土壤中富集了原油降解微生物菌群。采用重量法分析了富集菌群的降解效率和原油族组分的变化情况, 并通过控制环境因素变化来研究菌群最佳降油条件和混合菌群的优势菌属. 实验结果表明, 分离得到的菌群显示出可靠的原油生物降解率, 而其混合物的原油生物降解效率30 d内高达(48.0±3.5)%. 反应过程中降解了11.1%的胶质、79.7%的饱和烃和45.7%的芳烃.原油生物降解的最佳反应条件为中性pH, 30 ℃且矿物盐浓度为0.8%~4.0%. 混合菌群中的优势属为Pseudomonas、Stenotrophomonas、Brucella、Serratia、Brevundimonass和Achromobacter, 在原油降解期间, 群落中物种的丰富度和多样性是稳定的. 因此, 从多个不同来源进行微生物富集以构建混合菌群用于原油污染生物修复是可行的.

References:

[1] Singh H, Bhardwaj N, Arya S K, et al. Environmental impacts of oil spills and their remediation by magnetic nanomaterials[J]. Environmental Nanotechnology, Monitoring and Management, 2020, 14: 100305. DOI: 10.1016/j.enmm.2020.100305.
[2] Obafemi Y D, Taiwo O S, Omodara O J, et al. Biodegradation of crude petroleum by bacterial consortia from oil-contaminated soils in Ota, Ogun State, South-Western, Nigeria[J]. Environmental Technology and Innovation 2018, 12: 230-242. DOI: 10.1016/j.eti.2018.09.006.
[3] Xue J, Yu Y, Bai Y, et al. Marine oil-degrading microorganisms and biodegradation process of petroleum hydrocarbon in marine environments: A review[J]. Current Microbiology, 2015, 71(2): 220-228. DOI: 10.1007/s00284-015-0825-7.
[4] Parach A, Rezvani A, Assadi M M, et al. Biodegradation of heavy crude oil using Persian Gulf autochthonous bacterium[J]. International Journal of Environmental Research, 2017, 11(5): 667-675. DOI: 10.1007/s41742-017-0059-6.
[5] Cui J, Huang L, Wang W, et al. Maximization of the petroleum biodegradation using a synthetic bacterial consortium based on minimal value algorithm[J].International Biodeterioration and Biodegradation, 2020, 150: 104964. DOI: 10.1016/j.ibiod.2020.104964.
[6] Poi G, Aburto-Medina A, Mok P C, et al. Large scale bioaugmentation of soil contaminated with petroleum hydrocarbons using a mixed microbial consortium[J]. Ecological Engineering, 2017, 102: 64-71. DOI: 10.1016/j.ecoleng.2017.01.048.
[7] Liu Y, Li C, Huang L, et al. Combination of a crude oil-degrading bacterial consortium under the guidance of strain tolerance and a pilot-scale degradation test[J].Chinese Journal of Chemical Engineering, 2017, 25(12): 1838-1846. DOI: 10.1016/j.cjche.2017.02.001.
[8] Khanpour-Alikelayeh E, Partovinia A, Talebi A, et al. Investigation of Bacillus licheniformis in the biodegradation of Iranian heavy crude oil: A two-stage sequential approach containing factor-screening and optimization[J]. Ecotoxicology and Environmental Safety, 2020, 205: 111103. DOI: 10.1016/j.ecoenv.2020.111103.
[9] National Energy Administration. Ananlysis method for family composition of rock extract and crude oil:SY/T 5119—2016[S]. Beijing: Petroleum Industry Press, 2016.(in Chinese)
[10] Chen W, Kong Y, Li J, et al. Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers[J]. International Biodeterioration and Biodegradation, 2020, 154: 105047. DOI: 10.1016/j.ibiod.2020.105047.
[11] Wang X, Cai T, Wen W, et al. Surfactin for enhanced removal of aromatic hydrocarbons during biodegradation of crude oil[J]. Fuel, 2020, 267: 117272. DOI: 10.1016/j.fuel.2020.117272.
[12] Minai-Tehrani D, Rohanifar P, Azami S. Assessment of bioremediation of aliphatic, aromatic, resin, and asphaltene fractions of oil-sludge-contaminated soil[J]. International Journal of Environmental Science and Technology, 2015, 12(4): 1253. DOI: 10.1007/s13762-014-0720-y.
[13] Kopytov M A, Filatov D A, Altunina L K. Biodegradation of high-molecular-mass heteroatomic components of heavy oil[J]. Petroleum Chemistry, 2014, 54(1): 58-64. DOI: 10.1134/S0965544113040087.
[14] Liu B, Liu J, Ju M, et al. Purification and characterization of biosurfactant produced by Bacillus licheniformis Y-1 and its application in remediation of petroleum contaminated soil[J]. Marine Pollution Bulletin, 2016, 107(1): 46-51. DOI: 10.1016/j.marpolbul.2016.04.025.
[15] Al-Hawash A B, Dragh M A, Li S, et al. Principles of microbial degradation of petroleum hydrocarbons in the environment[J]. The Egyptian Journal of Aquatic Research, 2018, 44(2): 71-76. DOI: 10.1016/j.ejar.2018.06.001.
[16] Qin X, Tang J C, Li D S, et al. Effect of salinity on the bioremediation of petroleum hydrocarbons in a saline-alkaline soil[J]. Letters in Applied Microbiology, 2012, 55(3): 210-217. DOI: 10.1111/j.1472-765X.2012.03280.x.
[17] Zhang X, Xu D, Zhu C, et al. Isolation and identification of biosurfactant producing and crude oil degrading Pseudomonas aeruginosa strains[J]. Chemical Engineering Journal, 2012, 209: 138-146. DOI: 10.1016/j.cej.2012.07.110.
[18] Tiwari B, Manickam N, Kumari S, et al. Biodegradation and dissolution of polyaromatic hydrocarbons by Stenotrophomonas sp.[J]. Bioresource Technology, 2016, 216: 1102-1105. DOI: 10.1016/j.biortech.2016.06.047.
[19] Witono B. Biodegradation of used synthetic lubricating oil by Brevundimonas diminuta AKL 1.6[J]. Makara Journal of Science, 2017, 21(3): 136-142. DOI: 10.7454/mss.v21i3.7382.
[20] Muthukumar N, Mohanan S, Maruthamuthu S, et al. Role of Brucella sp. and Gallionella sp. in oil degradation and corrosion[J]. Electrochemistry Communications, 2003, 5(5): 421-425. DOI: 10.1016/S1388-2481(03)00093-6.
[21] Huang Y, Zhou H, Zheng G, et al. Isolation and characterization of biosurfactant-producing Serratia marcescens ZCF25 from oil sludge and application to bioremediation[J]. Environmental Science and Pollution Research, 2020, 27(22): 27762. DOI: 10.1007/s11356-020-09006-6.
[22] Li X, Zhao L, Adam M. Biodegradation of marine crude oil pollution using a salt-tolerant bacterial consortium isolated from Bohai Bay, China[J]. Marine Pollution Bulletin. 2016, 105(1): 43-50. DOI: 10.1016/j.marpolbul.2016.02.073.

Memo

Memo:

Biographies: Zhu Chongzhi(1996—), male, graduate; Yu Ran(corresponding author), female, doctor, professor, yuran@seu.edu.cn.
Foundation item: The National Natural Science Foundation of China(No.51878145), the National Key R&D Program of China(No.2018YFC1803100), the Key Research and Development Program of Department of Science and Technology of Jiangsu Province(No.BE2019709), the Six Talent Peaks Project of Jiangsu Province(No.JNHB-010).
Citation: Zhu Chongzhi, Zhang Xiaodong, Sun Liwei, et al. Development and characterization of a functional microbial consortium for crude oil degradation[J].Journal of Southeast University(English Edition), 2021, 37(4):.388-393.DOI:10.3969/j.issn.1003-7985.2021.04.007.

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